Canine Amyloid-Beta(1-42) ELISA Kits. Part Nos. cABeta1-42 & c2ABETA1-42-ELISA . KW canine beta-amyloid 1-42, canine AB1-42

Canine Amyloid-Beta (1-42;  Abeta1-42, AB1-42) ELISA Assay Kit

 

Dimentia Disease and Amyloid Synthesis and Degradation Pathway ELISA Kit Product Line

• Fully pre-configured sandwich ELISA format providing convenience and high sensitivity
  
• Strip plate format allowing separate use and storage of ELISA plate sections
• Affinity purified monoclonal capture antibody on plate.  Separate biotin-conjugated detection antibody
• Colorimetric detection
  
• Rapid Assay: 1.5 hr total incubation at 37^oC.
  
• Suitable for CSF, other biological fluids, cell and tissue homogenates and lysates
• ELISA kits are also available for human and rat Abeta1-42;  please inquire.

(Lead time:  2 weeks.  Use the protocol shipped with the kit for your experiment).

 

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Specifications:

 

Standard Concentration:  1280pg/ml

Assay Range:  10pg/ml→1000pg/ml

Sensitivity:  6.125pg/ml  

 

   View Protocol

 

1. 8 x 12 well removable section strip plate coated with antibody
2. Polyclonal detection antibody conjugated with biotin
3. Steptavidin-HRP
4. Protein Standard
5. Standard diluent
6. Concentrated wash solution
7. Chromogen solutions A and B
8. Stop solution
9. Plate sealers

 

For canine Aß(1-40) ELISA Kit, click here

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Background on Protein Targets of the Amyloid ELISA Kits

Amyloid-Beta:  Aged canines accumulate human-type amyloid-beta peptide and undergo declines in cognitive function^1,2.  Amyloid-beta concentrations in cerebral-spinal fluid appear to reflect amyloid-beta accumulation in brain^1,2.   Amyloid-beta peptides, and secretases in canine are similar to human¹.  Therefore, canine amyloid-beta accumulation and decline in cognitive function are being employed as an animal model for the onset of dimentia disease in humans. 

Amyloid-Beta (Aβ) is generated by sequential cleavage by beta and gamma secretases of the amyloid precursor protein (APP), a transmembrane protein of unknown function.  Inhibition of secretase activity has been proposed as a therapeutic strategy to limit amyloid-Beta (Aβ) accumulation. The γ secretase, which produces the C-terminal end of the Aβ peptide, cleaves within the transmembrane region of APP and generates amyloid-Beta (Aβ) isoforms of 36-43 amino acid residues in length.  The most common isoforms of amyloid-Beta are Aβ₄₀ and Aβ₄₂.  The shorter amyloid-Beta isoform is produced by cleavage in the endoplasmic reticulum, while the longer isoform is processed in the trans-Golgi network.  Aβ₄₀ is the most common amyloid-Beta (Aβ) isoform, but Aβ₄₂ is the more fibrillogenic and thus the predominant form associated Alzheimer’s disease.  Antibodies employed in this Amyloid-Beta ELISA kit are selective for the Aβ₄₂ isoform of amyloid-beta, with insignificant cross-reactivity to the Aβ₄₀isoform.

1. Head E, Pop V, Sarsoza F, Kayed R, Beckett TL, Studzinski CM, Tomic JL, Glabe CG, Murphy MP.  2010.  Amyloid-beta peptide and oligomers in the brain and cerebrospinal fluid of aged canines.  J Alzheimers Dis. 2010;20(2):637-46.
   
2. Cummings BJ,  Su JH, Cotman CW, White R, Russel MJ.  1993.  Beta-amyloid accumulation in aged canine brain: a model of early plaque formation in Alzheimer's disease. Neuro Aging. 1993 Nov-Dec;14(6):547-60.
   

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Methods Overview:

  Representative Sandwich ELISA Methods   

                                                       ELISA Assay Steps

Safety, Storage, Shipping

Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.