Canine Amyloid-Beta ELISA Kits (1-40). Part No. c2ABETA1-40-ELISA , kw. beta amyloid, ABeta, AB

• Fully pre-configured sandwich ELISA format providing convenience and high sensitivity
  
• Strip plate format allowing separate use and storage of ELISA plate sections
• Affinity purified monoclonal capture antibody on plate.  Separate biotin-conjugated detection antibody
• Colorimetric detection
  
• Rapid Assay: 1.5 hr total incubation at 37^oC.
  
• Suitable for CSF, other biological fluids, cell and tissue homogenates and lysates
• ELISA kits are also available for human and rat Abeta1-40;  please inquire.

1. 8 x 12 well removable section strip plate coated with antibody
2. Polyclonal detection antibody conjugated with biotin
3. Steptavidin-HRP
4. Protein Standard
5. Standard diluent
6. Concentrated wash solution
7. Chromogen solutions A and B
8. Stop solution
9. Plate sealers

Background on Protein Targets of the Amyloid ELISA Kits

Amyloid-Beta:  Aged canines accumulate human-type amyloid-beta peptide and undergo declines in cognitive function^1,2.  Amyloid-beta concentrations in cerebral-spinal fluid appear to reflect amyloid-beta accumulation in brain^1,2.   Amyloid-beta peptides, and secretases in canine are similar to human¹.  Therefore, canine amyloid-beta accumulation and decline in cognitive function are being employed as an animal model for the onset of dimentia disease in humans. 

Amyloid-Beta (Aβ) is generated by sequential cleavage by beta and gamma secretases of the amyloid precursor protein (APP), a transmembrane protein of unknown function.  Inhibition of secretase activity has been proposed as a therapeutic strategy to limit amyloid-Beta (Aβ) accumulation. The γ secretase, which produces the C-terminal end of the Aβ peptide, cleaves within the transmembrane region of APP and generates amyloid-Beta (Aβ) isoforms of 36-43 amino acid residues in length.  The most common isoforms of amyloid-Beta are Aβ₄₀ and Aβ₄₂.  The shorter amyloid-Beta isoform is produced by cleavage in the endoplasmic reticulum, while the longer isoform is processed in the trans-Golgi network.  Aβ₄₀ is the most common amyloid-Beta (Aβ) isoform, but Aβ₄₂ is the more fibrillogenic and thus the predominant form associated Alzheimer’s disease.  Antibodies employed in this Amyloid-Beta ELISA kit are selective for the Aβ₄₂ isoform of amyloid-beta, with insignificant cross-reactivity to the Aβ₄₀isoform.

1. Head E, Pop V, Sarsoza F, Kayed R, Beckett TL, Studzinski CM, Tomic JL, Glabe CG, Murphy MP.  2010.  Amyloid-beta peptide and oligomers in the brain and cerebrospinal fluid of aged canines.  J Alzheimers Dis. 2010;20(2):637-46.
   
2. Cummings BJ,  Su JH, Cotman CW, White R, Russel MJ.  1993.  Beta-amyloid accumulation in aged canine brain: a model of early plaque formation in Alzheimer's disease. Neuro Aging. 1993 Nov-Dec;14(6):547-60.
   

Methods Overview:

  Representative Sandwich ELISA Methods   

                                                        ELISA Assay Steps

1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37^oC.  Wash 5 times.
3. Apply chromogens, incubate, then stop reaction.