PROTEASE INHIBITOR COCKTAIL w/ EDTA (100X, 1ml; PI-2)

PROTEASE INHIBITOR COCKTAIL w/ EDTA (100X, 1ml;  PI-2)
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Price: $115.95
Availability: Out Of Stock
Model: PI-2
Manufacturer: Fivephoton Biochemicals

Protease Inhibitor Cocktail with EDTA 100X, Water Soluble

(Makes a 1 ml 100x protease inhibitor cocktail solution when suspended in dH20.  Provided lyophilized)
  • Broad spectrum and water soluble protease inhibitor cocktail.
  • Protease inhibitor cocktail includes inhibition of serine and cysteine proteases and aminopeptidases.
  • Inhibits calcium dependent proteases.
  • Suspend in dH20.  Add aliquot of 100X Protease Inhibitor Cocktail directly to cell lysates.

Directions:  Suspend lyophilized material in 1 ml dH20 to make 100X solution.  Store aliquots at -20 degrees C.



Protease Inhibitor Cocktail with EDTA Contents (at 100X concentration) 
  1. AEBSF;  50mM.  
  2. Aprotinin;  30 uM.  
  3. E-64;  1 mM.  
  4. Bestatin;  1mM.  
  5. Leupeptin;  1mM. 
  6. EDTA;  5mM.
  Specification Sheet



Related Product:  Protease Inhibitor Cocktail w/o EDTA, water soluble:  click here


Protease Inhibitor References
  1. Arai T, Ikarashi Y, Okamoto K, Kuribara H, and Maruyama Y. Memory disturbance and hippocampal degeneration induced by continuous intraventricular infusion of a protease inhibitor, leupeptin. Brain Res 754: 157-162, 1997.
  2. Christoffers KH, Khokhar A, Chaturvedi K, and Howells RD. Inhibition of mu and delta opioid receptor ligand binding by the peptide aldehyde protease inhibitor, leupeptin. Regul Pept 105: 9-14, 2002.
  3. Citron M, Diehl TS, Capell A, Haass C, Teplow DB, and Selkoe DJ. Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF. Neuron 17: 171-179, 1996.
  4. Hussein EM, Dawoud HA, Salem AM, and Atwa MM. Antiparasitic activity of cystine protease inhibitor E-64 against Giardia lamblia excystation in vitro and in vivo. J Egypt Soc Parasitol 39: 111-119, 2009.
  5. Jose J and Zangen D. Autodisplay of the protease inhibitor aprotinin in Escherichia coli. Biochem Biophys Res Commun 333: 1218-1226, 2005.
  6. Nakabo Y and Pabst MJ. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. J Leukoc Biol 60: 328-336, 1996.
  7. Rudzinski T, Mussur M, Gwiazda Z, and Mussur M. Protease inhibitor leupeptin attenuates myocardial stunning in rat heart. Med Sci Monit 10: BR4-10, 2004.
  8. Sekiguchi T, Hosoyama Y, and Miyata S. Effects of proteasome inhibitor (lactacystin) and cysteine protease inhibitor (E-64-d) on processes of mitosis in Xenopus embryonic cells. Zoolog Sci 19: 1251-1255, 2002.
  9. Tissot G, Canard H, Nadai M, Martone A, Botterman J, and Dubald M. Translocation of aprotinin, a therapeutic protease inhibitor, into the thylakoid lumen of genetically engineered tobacco chloroplasts. Plant Biotechnol J 6: 309-320, 2008.
  10. Tsuji S and Matsushita H. Successful treatment of murine muscular dystrophy with the protease inhibitor bestatin. J Neurol Sci 72: 183-194, 1986.
  11. Vlasova TI and Vanyushin BF. DNA methylation by wheat cytosine DNA methyltransferase: modulation by protease inhibitor E-64. Biochem Mol Biol Int 45: 145-153, 1998.

Protease Inhibitor Cocktail Inhibitor with EDTA:   Storage, Safety, Shipping.
Storage:  Lyophiilized material ships at ambient temperature.  Store at -20 degrees C upon arrival.   -20 degrees C when diluted in dH20.
Safety:  Harmful.  Avoid ingestion, eye and skin contact.  Wear protective clothing, gloves and eye protection.

Shipping options for Protease Inhibitor Cocktail:  Two day domestic delivery (Shipping charges:  $15.00).  Domestic overnight Fedex delivery (shipping charges $27.00).  International delivery is also available.

PROTEASE INHIBITOR, COCKTAIL, AEBSF, APROTININ, E-64, LEUPEPTIN, BESTATIN


 

Category Download Link
MSDS click here
Specification Sheet click here