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HUMAN KYNURENIC KYNA ELISA KIT (96T: Part hKYNA-ELISA)

HUMAN KYNURENIC KYNA ELISA KIT (96T: Part hKYNA-ELISA)
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Price: $645.00
Availability: In Stock
Model: hKYNA-ELISA

DetailsManuals & Downloads
 
KYNURENIC (KYNA) ELISA Kit 
Sandwich ELISA format (96T)

Highlights
  • Assay ranges:  0.05nmol/ml→15nmol/ml
  • 0.038nmol/ml sensitivity
  • Sandwich ELISA format providing convenience and high sensitivity. 
  • Strip plate format allowing separate use and storage of sections of the ELISA plate.
  • Rapid protocol:  Only one incubation with sample to antibodies.  Total protocol time approximately 1.5 hrs.
  • Colormetric detection at 450nm using a standard absorbance plate reader.
Lead time 2 weeks.


Price (USD)
 Number of Kits 
 Number of Assays
$645 1
 96T



 
 
Specifications 
Species
 Part
 Standard
 Assay Range
 Sensitivity
Human
 hKYNA-ELISA
 16nmol/ml
 0.05nmol/ml - 15nmol/ml
 0.038 nm/ml

 

                               
 
 
 
Kit Contents
  1. 8 x 12 well removable section strip plate coated with anti-KYNA antibody
  2. Anti-KYNA polyclonal detection antibody conjugated with biotin
  3. Steptavidin-HRP
  4. Protein Standard
  5. Standard diluent
  6. Concentrated wash solution
  7. Chromogen solutions A and B
  8. Stop solution
  9. Plate sealers
Kynurenic acid (KYNA or KYN) is a product of L-tryptophan metabolism. Kynurenic acid (KYNA) has neuroactive activity by operating as an antagonist to excitatory amino acid receptors.  Therefore, elevated Kynurenic acid (KYNA) concentration could modulate neurological abnormalities.  Some studies have indicated it has altered concentrations in CSF of Alzheimer's patients, and could potentially serve as a biomarker and/or modulator of the disease.  
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.  
 

   Sandwich ELISA Methods for KYNA                                                                                                 

ELISA Assay Steps
  1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.

Safety, Storage, Shipping, Kynurenic Acid ELISA Kit
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4oC.  Expires 6 months after manufacture.
Shipping.  Overnight domestic delivery recommended.  Also available for international delivery.  Please inquire for rates.


 

Category Download Link
Protocol Manual click here
MSDS click here