RAT TNF ALPHA ELISA KIT (TNFa, TNF-ALPHA, Part rTNFalpha-ELISA)
Click to enlarge |
| Price: |
$625.00 |
| Availability: |
In Stock |
| Model: |
rTNFalpha-ELISA |
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Rat TNF Alpha ELISA Kit
 Protocol
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Product name |
Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
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|
Part No |
rTNFalpha-ELISA |
|
Detection method |
Sandwich ELISA, Double Antibody
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|
Size |
96T
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|
Range |
15.625-1000pg/ml
|
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Sensitivity |
< 9.375pg/ml
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|
Standard curve |
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Storage |
4 ℃ for 6 months
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Recovery |
Matrices listed below were spiked with TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum (n=5) |
87-103 |
94 |
|
EDTA plasma (n=5) |
91-105 |
97 |
|
heparin plasma (n=5) |
85-101 |
95 |
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Linearity |
The linearity of the kit was assayed by testing samples spiked with TNF-α and their serial dilutions. The results are displayed by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum (n=5) |
90-103% |
87-102% |
86-99% |
85-101% |
|
EDTA plasma (n=5) |
86-96% |
83-101% |
82-101% |
84-101% |
|
heparin plasma (n=5) |
84-98% |
86-96% |
85-98% |
85-95% |
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|
CV(%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10%
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|
Note |
For Research Use Only
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Shipping: Styrofoam with ice pack.
Storage: 4oC, 6 months
|
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