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Monoamine Oxidase B (MAO-B) ELISA Kits
Highly sensitive sandwich ELISA Kits with pg/ml detection and rapid protocol. 96T
Lead time: 2 weeks.
Monoamine Oxidase B (MAO-B) ELISA Kits. Separate kits are available for human, mouse, & rat
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Fully pre-configured sandwich ELISA format providing optimal convenience, specificity and sensitivity
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Strip plate configuration allowing separate use and storage of 96-well ELISA plate sections
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Affinity purified polyclonal or monoclonal capture antibodies coated on plate, depending on kit format. Separate HRP-conjugated detection antibody
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Rapid colorimetric detection. 1 hr total incubation at 37oC
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Suitable for cell and tissue lysates and homogenates
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Highly sensitive: pg/ml detection
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Available for Human, mouse, rat, and porcine MAO-B
Protocol MAO-B ELISA Format 1
Protocol MAO-B ELISA Format 2
Specifications of Monoamine Oxidase B ELISA Kits
Format 1.
Species
|
Standard
|
Assay Range
|
Price
|
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Human |
96 ng/ml |
0.3 ng/ml - 90 ng/ml
|
$590 |
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Mouse |
16 ng/ml |
0.1 ng/ml - 15 ng/ml |
$620 |
|
Rat |
96 ng/ml |
0.3 ng/ml - 90 ng/ml
|
$620 |
|
Porcine |
96 ng/ml |
0.3 ng/ml - 90 ng/ml |
$640 |
Format 2.
Species
|
Standard
|
Assay Range
|
Price |
|
Human |
360 ng/ml |
3 ng/ml - 300 ng/ml |
$590 |
|
Rat |
180 ng/ml |
1.5 ng/ml - 150 ng/ml |
$620 |
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Mouse |
180 ng/ml |
1.5 ng/ml - 150 ng/ml |
$620 |
|
Porcine |
1.8 ng/ml |
20 pg/ml - 1.2 ng/ml |
$640 |
Use Available Options menu to select kit and species.
Background: Monoamine Oxidase B (MAO-B) is involved in the degradation of dopamine. It is mostly found in the outer mitochondrial membrane of neural and cardiac cells. MAO-B levels may increase with age, suggesting a role in age related cognitive decline and the increased likelihood of developing neurological diseases later in life. Polymorphisms of the MAOB gene have been linked to negative emotionality, and suspected as a factor in depression. High levels of MAO-B in the brain are correlated with Parkinson's Disease and Alzheimers. Activity of MAO-B has also been shown to play a role in stress-induced cardiac damage. Monoamine oxidase is the target of inhbitors treating neurological disorders.
ELISA kits for Monoamine Oxidase A (MAOA) are also available: click here
Deamination reaction catalyzed by Monoamine Oxidase
Methods Overview
Sandwich ELISA Format 1: The ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with provided biotin-linked detection antibody and streptavidin-HRP solutions, which are then added to the plate and incubated for 60min. The plate is washed and color reagents are added, resulting in a reaction with HRP. A stop solution is applied and the resulting yellow solution is measured at 450nm.
Sandwich ELISA Format 2: The included ELISA 96-well micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm. This assay requires approximately 2.5 hrs to complete at RT.
*(Use the protocol shipped with the kit for your experiment).
Representative Sandwich ELISA Methods
Steps of Sandwich ELISA Format 1
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Mix sample, provided biotinylated-detection antibody and provided streptavidin-HRP.
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Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC. Wash 5 times.
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Apply chromogens, incubate, then stop reaction.
Steps of Sandwich ELISA Format 2
1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add the stop solution
References on Monoamine Oxidase B:
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Glover, V., Sandler, M., Owen, F., & Riley, G. J. (1977). Dopamine is a monoamine oxidase B substrate in man.
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Kurth, J. H., Kurth, M. C., Poduslo, S. E., & Schwankhaus, J. D. (1993). Association of a monoamine oxidase B allele with Parkinson's disease. Annals of neurology, 33(4), 368-372.
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Binda, C., Li, M., Hubálek, F., Restelli, N., Edmondson, D. E., & Mattevi, A. (2003). Insights into the mode of inhibition of human mitochondrial monoamine oxidase B from high-resolution crystal structures. Proceedings of the National Academy of Sciences, 100(17), 9750-9755.
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Binda, C., Newton-Vinson, P., Hubálek, F., Edmondson, D. E., & Mattevi, A. (2002). Structure of human monoamine oxidase B, a drug target for the treatment of neurological disorders. Nature Structural & Molecular Biology, 9(1), 22-26
For research only. Not for diagnostics of disease in humans or animals.
Lead time: approximately 2 weeks.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC, 6 months. Longer term storage of sections of the strip plate and standards at -20oC. Seal and store 96-well ELISA plate in a zip locks bag after opening.
Shipping. Overnight domestic delivery recommended (Flat rate Fedex cost for continental US: $52). Also available for international delivery; please inquire for shipping cost.
KW: Monoamine Oxidase B ELISA Kit
Human Monoamine Oxidase B ELISA Kit
Mouse Monoamine Oxidase B ELISA Kit
Rat Monoamine Oxidase B ELISA Kit
Porcine Monoamine Oxidase B ELISA Kit
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