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InformationRat trkA ELISA Kit (Part FPEK0847)
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Rat trkA ELISA Kit (96T)
Highly sensitive kit to rat trkA. Validated for sensitive and selective detection in cell culture medium, serum and plasma.
Currently unavailable. Call for availability of substitute kit to same target.
Highlights
- Fully configured colorimetric ELISA kit in sandwich fomat.
- Natural - purified protien provided as standard.
- Assay range: 156pg/ml-10,000pg/ml.
- Validated for cell culture medium, serum and plasma.
View protocolKit Contents
| Description | Quantity |
| 96- well plate precoated with anti-rat trkA antibody | 1 |
| Lyophilized natural rat trkA standard | 10ng (1 vial) |
| Biotinylated anti-rat trkA Antibody | 130 ul (dilution 1:100) |
| Sample diluent buffer |
30 ml |
| Antibody diluents buffer |
12 ml |
| ABC diluents buffer |
12 ml |
| TMB color developing agent |
10 ml |
| TMB stop solution |
10 ml |
trkA ELISA Kits Availability And Specifications
|
Species |
Assay Range |
| Human | 156pg/ml-10,000pg/ml |
| Rat | 156pg/ml-10,000pg/ml |
| Mouse | 156pg/ml-10,000pg/ml |
Use Available Options menu to select the species specificity of the trkA ELISA Kits.
Background on trkA
trkA is a protein encoded by the NTRK1 gene. The trkA kinase is a high affinity membrane bound receptor for Nerve Growth Factor, that phosphorylates itself and members of the MAPK pathway when a neurotrophin binds. It's presence leads to cell differentiation and specificity of sensory neuron subtypes. Mutations of this gene lead to self-mutilating behavior, mental retardation, and cancer.
Methods Overview: Sandwich ELISA
This rat trkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for trkA has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for trkA is added subsequently and then followed by washes with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of yellow solution is proportional to the amount of rat trkA captured on the plate.
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Representative Sandwich ELISA Methods |
- Add sample to plate, incubate 90 min, 37oC.
- Add detection antibody, incubate 1 hr, 37oC.
- Add ABC solution, incubate 30 min 37oC.
- Add chromogens, incubate in dark for 25 min.
- Add stop solution. Measure OD at 450nm.
References
1. Castañeda-Corral G, Jimenez-Andrade JM, Bloom AP, Taylor RN, Mantyh WG, Kaczmarska MJ, Ghilardi JR, Mantyh PW. Neuroscience. 2011 Mar 31;178:196-207. Epub 2011 Jan 26.
2. Georgieva MV, de Pablo Y, Sanchis D, Comella JX, Llovera M. J Neurochem. 2011 Feb 19.
3. Benito-Gutiérrez E, Garcia-Fernàndez J, Comella JX (February 2006). "Origin and evolution of the Trk family of neurotrophic receptors". Mol. Cell. Neurosci. 31 (2): 179-92.
4. Lambiase A, Merlo D, Mollinari C, Bonini P, Rinaldi AM, D' Amato M, Micera A, Coassin M, Rama P, Bonini S, Garaci E (November 2005). "Molecular basis for keratoconus: lack of TrkA expression and its transcriptional repression by Sp3". Proc. Natl. Acad. Sci. U.S.A. 102 (46): 16795-800.
Safety: Stop solution contains acid. Avoid eye contact. Wear eye protection and gloves.
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Shipping. Overnight delivery