Home
Products
Manuals
Log In
Basket
Checkout
RSS
Contacts
Categories
InformationRABBIT, RAT or MOUSE ALPHA-SYNUCLEIN (Α-SYNUCLEIN, ALPHA SYNUCLEIN, SNCA; RABBIT) HIGH SENSITIVITY ELISA ASSAY KIT (PART NO. RSNCA-ELISA)
 Click to enlarge |
|
Mouse, Rat or Rabbit Alpha-Synuclein (α-Synuclein, SNCA) High Sensitivity ELISA Assay Kit (96T)
Lead Time: Approx 2 weeks.
- Fully pre-configured sandwich ELISA format providing technical convenience and high sensitivity
- Strip plate cassette configuration allowing separate use and storage of ELISA plate sections
-
Detection Range: 3 pg/ml -135 pg/ml for mouse, rat or rabbit
-
Standard Peptide Concentration: 135pg/ml
-
Affinity purified polyclonal capture antibody coated on plate. Separate HRP-conjugated detection antiobody
- Rapid colorimetric detection. 1 hr total incubation at 37oC; 2 hr incubation at RT
View General Protocol
|
Price (96T; USD) |
No. of Kits |
|
$635 |
1 |
|
$1150.00 |
2 |
|
$2100.00 |
4 |
Methods Overview:
The included ELISA 96-well micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal capture antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added which upon conversion by HRP become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm.
This ELISA assay kit provides a standard peptide solution with known concentration to calibrate absorbance readings to concentration.
Representative Sandwich ELISA Methods to Alpha-Synuclein (SNCA)
Steps of Sandwich α-Synuclein ELISA
1. Equilibrate plate to ambient temperature
1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add the stop solution
Background: Alpha-Synuclein: Alpha-synuclein (SNCA gene) is a 140 amino acid protein expressed in brain that is associated with fibrillar structures in Lewy Bodies. Although primary located in the cytosol, alpha-synuclein binds to phospholipid and may assemble into pore-like structures in cellular membranes. Generation of aggregates in Lewy Bodies and/or association of mutant or wild-type alpha-synuclein with membraneous structures have been attributed to the etiology of both early-onset congenital and age-related Parkinson’s Disease.
Parkinson's disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features of the disease include the characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies in brain and spinal cord.
References on Alpha-Synuclein
- Adamczyk, A. et al. (2007) Alpha-synuclein decreases arachidonic acid incorporation into rat striatal synaptoneurosomes. Folia Neuropathol 45 (4), 230-235
- Kim, H.J. et al. (2007) Seed-dependent accelerated fibrillation of alpha-synuclein induced by periodic ultrasonication treatment. J Microbiol Biotechnol 17 (12), 2027-2032
- Wakabayashi, K. et al. (2007) The Lewy body in Parkinson's disease: molecules implicated in the formation and degradation of alpha-synuclein aggregates.Neuropathology 27 (5), 494-506
- Yoshida, M. (2007) Multiple system atrophy: alpha-synuclein and neuronal degeneration. Neuropathology 27 (5), 484-493
- Kloepper, K.D. et al. (2007) Solid-state NMR spectroscopy reveals that water is nonessential to the core structure of alpha-synuclein fibrils. J Phys Chem B111 (47), 13353-13356
- Buchman, V.L. and Ninkina, N. (2008) Modulation of alpha-synuclein expression in transgenic animals for modelling synucleinopathies--is the juice worth the squeeze? Neurotox Res 14 (4), 329-341
- Fuchs, J. et al. (2008) Genetic variability in the SNCA gene influences alpha-synuclein levels in the blood and brain. Faseb J 22 (5), 1327-1334
- Parihar, M.S. et al. (2009) Alpha-synuclein overexpression and aggregation exacerbates impairment of mitochondrial functions by augmenting oxidative stress in human neuroblastoma cells. Int J Biochem Cell Biol 41 (10), 2015-2024
- Gibrat, C. et al. (2009) Differences between subacute and chronic MPTP mice models: investigation of dopaminergic neuronal degeneration and alpha-synuclein inclusions. J Neurochem 109 (5), 1469-1482
- Kim, S. et al. (2009) Alpha-synuclein induces migration of BV-2 microglial cells by up-regulation of CD44 and MT1-MMP. J Neurochem 109 (5), 1483-1496
- Leong, S.L. et al. (2009) Modulation of alpha-synuclein aggregation by dopamine: a review. Neurochem Res 34 (10), 1838-1846
- Xilouri, M. et al. (2009) Abberant alpha-synuclein confers toxicity to neurons in part through inhibition of chaperone-mediated autophagy. PLoS One 4 (5), e5515
- Noguchi-Shinohara, M. et al. (2009) CSF alpha-synuclein levels in dementia with Lewy bodies and Alzheimer's disease. Brain Res 1251, 1-6
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC.
Shipping. Overnight domestic delivery recommended with ice pack (cost: $52 for 1-2 kits). Also avaialble for international delivery; please inquire for shipping cost.
Related Research Tool. Fluorescent Dopamine (Part DnsylD-1). Dopamine conjugated with the fluorescent tag dansyl through a 5-carbon polylinker. This fluorescent compoud manufactured exclusively by Fivephoton Biochemicals maintains the biochemical and pharmacological characteristics of native dopamine allowing effective application in transporter and ligand binding assays as a fluorescent tracer. The dansyl fluorescent tag produces a bright blue fluorescence with a large stokes shift between Ex and Em, which minimizes autofluorescence and non-specific signal.
