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PARKIN (E3 UBIQUITIN LIGASE, PARK2), PARKINSON'S DISEASE PROTEIN 2 ELISA KIT (Part No. Prk-ELISA) 96T

PARKIN (E3 UBIQUITIN LIGASE, PARK2), PARKINSON'S DISEASE PROTEIN 2 ELISA KIT (Part No. Prk-ELISA) 96T
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Price: $635.00
Availability: In Stock
Model: Prk-ELISA (96T)
Manufacturer: Fivephoton Biochemicals

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Select Species:
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Parkin (Ligase; PARK2) ELISA Kit 

Synonyms:  Park2,  Parkinson's Disease Protein 2
Highly Sensitive pg/ml detection
Currently unavailable

Separate kits are available for human, rat, mouse and canine.   

Lead time:  approximately 2 weeks

  • Fully pre-configured sandwich ELISA format providing convenience, and optimal selectivity and sensitivity.
  • Affinity purified polyclonal capture antibody coated on plate.  Separate HRP-conjugated detection antibody.
  • Colorimetric detection at 450 nM
  • Rapid assay format.  Total incubation time at 37oC is 1 hr.  
  View general protocol  

Price (USD)
 Number of Kits 
 Number of Assays
$635.00
 1
 96

                            
 
Specifications:
 
Species
 Standard Peptide
 Assay Range
Human
 135 pg/ml
 3 - 100 pg/ml
Mouse
 90 pg/ml
 2 - 70 pg/ml
Rat
 90 pg/ml
 2 - 70 pg/ml
Canine
 108 pg/ml
 3 - 90 pg/ml

 


                               
 
 
 
*  Use Available Options Menu to select species
Defects in PARK2 are the cause of Parkinson disease type 2 (PARK2) also known as early-onset parkinsonism with diurnal fluctuation (EPDF), or autosomal recessive juvenile Parkinson disease (PDJ), a neurodegenerative disorder characterized by bradykinesia, rigidity, postural instability, tremor, and onset usually before age 40.  This genetic disease differs from classic Parkinson disease by early DOPA-induced dyskinesia, diurnal fluctuation of the symptoms, sleep benefit, dystonia and hyper-reflexia.  Patients with PARK2 mutations show loss of dopaminergic neurons in the substantia nigra, similar to that seen in classic Parkinson disease, however, Lewy bodies (intraneuronal accumulations of aggregated proteins) are absent.

Image:  Ubiquitin-like domain of PARK2.  Source:  PDB:  
Methods Overview:  Sandwich ELISA

The included ELISA 96-well micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen.  Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support.  After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period.  Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored.  A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration.  An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm.  This assay requires approximately 1.5 hrs to complete at 37oC.


This ELISA assay kit provides a standard peptide solution with known concentration to calibrate absorbance readings to concentration. 
   Representative Sandwich ELISA Methods   

Steps Of Representative Sandwich ELISA Kit
1.  Equilibrate plate to ambient temperature
2.  Add sample, incubate 30 min - 1hr, wash 
3.  Add HRP-Antibody, incubate 30 min - 1 hr, wash
4.  Add the color reagent, incubate 15 min
5.  Add the stop solution


Selected References on Parkin
  1. Xabier Agirre, et.al. (2006).   Abnormal methylation of the common PARK2 and PACRG promoter is associated with downregulation of gene expression in acute lymphoblastic leukemia and chronic myeloid leukemia. International journal of cancer Journal international du cancer 118 (8) p. 1945-1953. 
  2. Nonmotor Symptoms in Patients with PARK2 MutationsAsako Yoritaka, Yumi Shimo, Yasushi Shimo, Yuichi Inoue, Hiroyo Yoshino, Nobutaka Hattori (2011). Parkinsons disease 2011 p. 47364. 
  3. George Poulogiannis, Rebecca E McIntyre, Maria Dimitriadi, John R Apps, Catherine H Wilson, Koichi Ichimura, Feijun Luo, Lewis C Cantley, Andrew H Wyllie, David J Adams, Mark J Arends (2010).  PARK2 deletions occur frequently in sporadic colorectal cancer and accelerate adenoma development in Apc mutant mice.
  4. Proceedings of the National Academy of Sciences of the United States of America 107 (34) p. 15145-15150.
  5. Yasuhiro Yamamura (2007).  The long journey to the discovery of PARK2.  Rinsho shinkeigaku Clinical neurology 47 (11) p. 749-751.

Safety, Storage, Shipping, Parkin ELISA Kit
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4oC.  Expires 6 months after manufacture.
Shipping:  Overnight domestic delivery recommended (Flat rate cost $52).  Also available for international delivery;  please inquire for international shipping rate.

kw.  PARKIN, UBIQUITIN LIGASE, PARK2, ELISA KIT, PARKINSON'S DISEASE


 

Category Download Link
Protocol Manual click here
MSDS click here



 

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