Rat Cyclophilin B (CYPB, PPIB, Peptidylprolyl Isomerase B) ELISA KITS Part No. CYPB-ELISA (r,2X96T)

Rat Cyclophilin B (CYPB, PPIB) ELISA Kit, 2X96T, 192 Assays

Lead time:  Approximately 2 weeks

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Synonyms:  PPIB; PPI-B; CYP-S1; CyP-B; SCYLP; Peptidylprolyl Isomerase B; Peptidyl-Prolyl Cis-Trans Isomerase B.  Separate Cyclophilin B (CYPB) kits are available for human, rat and mouse (see Related Products tab).

*Must be ordered in sets of 2 kits.  Enter 1 for a set of 2 Kits, enter 2 for a set of 4 kits, etc

Overview:  Cyclophilin B (CyPB) is a cyclosporine-binding protein located mainly within the endoplasmic reticulum.  It has also been detected in the nucleus where it may maintain a gene regulatory role.  Cyclophilin B has a signal sequence and is therefore associated with the secretory pathway and released in biological fluids. 

Given that Cyclophilin B is a soluble protein in the secretory pathway, the primary locations to detect and assay are in biological fluids, such as serum and plasma, cell culture supernatant, and cell and tissue lysates.   Use the isolation methods suggested in the protocol manual to prepare samples using biological fluids and cell culture supernatant.  To assay in cell or tissue lysates, use a non-denaturing lysis buffer to solubilize membranes and suspend intracellular Cyclophilin B into the lysis buffer supernatant.  The  protocol associated with the FIVEphoton Biochemicals ELISA Lysis and Protein Extraction Buffer (Part No. ELSP-1) provides detailed instructions how to isolate intracellular proteins by cell lysis in preparation for ELISA. 

Highlights of the Cyclophilin B (CyPB) ELISA Kit

1. Fully pre-configured kit in sandwich ELISA format
   
2. Affinity purified polyclonal capture antibody on plate.  HRP-conjuated detection antibody
3. Separate kits are offered for human (h), rat (r) and mouse (m).
   
4. Incubation Time.  First incubation:  1 hr at ambient temperature;  Second incubation with anti-p65 HRP;  1 hr at ambient temperature.  Alternatively, 30 min incubations can be performed at 37^oC for both steps.
   
5. Detection Method:  Colorimetric at 450nm
   

Protocol Synopsis of Sandwich Cyclophilin B (CyPB) ELISA