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TMPRSS2, TRANSMEMBRANE SERINE PROTEASE 2 ELISA Kit (Parts m, h TMPRSS2-ELISA 1)

TMPRSS2, TRANSMEMBRANE SERINE PROTEASE 2 ELISA Kit (Parts m, h TMPRSS2-ELISA 1)
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Price: $655.00
Availability: In Stock
Model: m, h TMPRSS2-ELISA 1, 2

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Species:
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TMPRSS2 Transmembrane Serine Protease 2 ELISA Kit 1 96T

TMPRSS2 is a type II transmembrane protein thought to be cleaved and secreted into cell media after autocleavage. TMPRSS2 is involved in viral entry by cleaving surface glycoproteins and priming for infection. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19). TMPRSS2 mediated cleavage in the S2 domain appears to be required for SARS-COV2 infection.  TMPRSS2 facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. 

Separate ELISA kits are available for human and mouse TMPRSS2
Lead Time:  Approximately 2 weeks
 
Highlights
  • Fully pre-configured ELISA kit in sandwich assay format.
  • Wide dynamic assay range: 80pg/ml - 20ng/ml depending on species.  Sensitivity: 20pg/ml.
  • Rapid ELISA Protocol:  Requires one 60 min incubation period at 37oC.
  • Samples are mixed with the provided biotinylated detection antibody and streptavidin-HRP prior to addition to plate.
Detection Characteristics

Human:  Assay range:  0.15 ng/ml - 32 ng/ml.  Sensitivity:  .137 ng/ml.  Standard Concentration:  40ng/ml. 
 
Mouse:   Assay range:  30 ng/ml- 7200 ng/ml;  Sensitivity:  24.6 ng/ml.  Standard Concentration:  8000 ng/ml.
 
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a provided biotin-linked detection antibody and provided streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.

   Sandwich ELISA Methods                                                       

ELISA Assay Steps
  1. Mix samples, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply samples and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.

 

Kit Contents
  1. 8 x 12 well removable section strip plate coated with anti-SNCB monoclonal antibody
  2. Anti-TMPRSS2 polyclonal detection antibody conjugated with biotin
  3. Steptavidin-HRP
  4. Protein Standard
  5. Standard diluent
  6. Concentrated wash solution
  7. Chromogen solutions A and B
  8. Stop solution
  9. Plate sealers
Safety, Storage, Shipping, TMRSS2 ELISA Kit
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4oC.  Expires 1 year after manufacture.
Shipping. Lead time 2 weeks.  Overnight domestic delivery recommended.  Also available for international delivery.  Please inquire for international delivery rates.
kw:  TMPRSS2, TRANSMEMBRANE SERINE PROTEASE 2, ELISA, ACE2


 

Category Download Link
Protocol Manual click here
MSDS click here