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InformationHuman Beta Klotho (KLB) ELISA Kit (96T, beta-klotho, hKLB-ELISA)
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Human beta-Klotho ELISA Kit
| Product name |
Human KLB(Beta-klotho) ELISA Kit |
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| Catalogue No. |
KLB-ELISA |
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| Alias |
KLB ELISA Kit, BKL(Beta-klotho) ELISA Kit, BetaKlotho ELISA Kit, Klotho beta-like protein ELISA Kit, klotho beta ELISA Kit, klotho beta like ELISA Kit |
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| Detection method |
Sandwich ELISA, Double Antibody |
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| Application |
KLB ELISA Kit allows for the in vitro quantitative determination of KLB concentrations in plasma, tissue homogenates and other biological fluids. |
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| Size |
96T |
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| Range |
15.6-1000pg/ml |
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| Sensitivity |
< 9.375pg/ml |
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| Standard curve |
 Human KLB(Beta-klotho) ELISA Kit |
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| Storage |
4 ℃ for 6 months |
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| Recovery |
Matrices listed below were spiked with certain level of KLB and the recovery rates were calculated by comparing the measured value to the expected amount of KLB in samples.
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| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) |
Intra-Assay: CV<8% |
Protocol
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Storage Micro ELISA Plate (Dismountable) 8×6 or 8×12 4°C/-20°C
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Lyophilized Standard 1 vial or 2 vial 4°C/-20°C Sample
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Standard dilution buffer 10ml/20ml 4°C
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Biotin-detection antibody (Concentrated) 60ul/120ul 4°C
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Antibody dilution buffer 5ml/10ml 4°C
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HRP-Streptavidin Conjugate(SABC) 60ul/120ul 4°C (light sensitive)
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SABC dilution buffer 5ml/10ml 4°C TMB substrate 5ml/10ml 4°C (light sensitive)
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Stop solution 5ml/10ml 4°C
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Wash buffer (25X) 15ml/30ml 4°C
Protocol Summary
