ALLOPHYCOCYANIN (APC) Fluorescent Protein (Part APC-1-Five)

ALLOPHYCOCYANIN Fluorescent Protein (Part APC-1-FIVE)

Allophycocyanin (APC) is a fluorescent protein with high absorbtivity and high quantum efficiency and can be conjugated to proteins for fluorescent detection. Allophycocyanin is attached to proteins using standard cross-linking techniques. Proteins conjugated with APC are used for cell sorting, flow cytometry, FRET, and ELISA. APC is isolated from cyanobacteria algae in the Spirulina genus.

10mg: $650

25mg: $1100

Bulk orders available: Please inquire

APC Specifications

Source: Cyanophyta'

Subunit Stucture: (αβ)₃

Molecular Weight: 105kDa

Absorption Maximum: 651 ± 2 nm

Emission Maximum: 660 ± 2 nm

Extinction Coefficient: 7.0 x 10⁵ cm^-1M^-1

Protein Concentration: ≧ 15g/mL

Purity: A651/A280 ≥ 5.0; A651/A620 ≥ 1.5

Stability: Supplied in 100 ml sodium phosphate buffer pH7.0 with 60% ammonium sulfate, 1mM EDTA, and 1mM sodium azide

Shelf Life: 2 years with proper storage

Storage: Store at in the dark at 4°C. Do not freeze. Composition: Liquid

Preparations Before Use:

APC is supplied and stored in ammonium sulfate solution. Therefore, APC needs to be re-suspended and dialyzed until no visible precipitates remain and ammonium sulfate is removed. Use PBS or your conjugation buffer (1 ml of APC to 1 liter of buffer) to dialyze.

Specification Sheet

Sample COA - Inquire for current lot

Storage: 4^oC. Avoid light exposure.

Shipping Options: Overnight with ice packs

Concentration	16.5 mg/ml. Sold in 5 mg quantitites
	Specification Values	Result Values
Purity	A650/ A280 ≥ 5.0	5.4
Contamination	A650/ A620 ≥ 1.5	1.52
	Cross-link ratio ≥ 1.2	1.3
Absorption Maximum	650 nm± 2nm	650
Emission Maximum	660 nm± 2nm	659
Storage	Store at 4°C, away from light. Do not freeze. For research or further manufacturing use only.
Form	Supplied in 100 mM sodium phosphate buffer pH 7.0 with 60 % ammonium sulfate, 1mM EDTA, and 1mM sodium azide.
Shelf Life	2 years under proper storage conditions.