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Mouse VEGF ELISA Kit (Part FPEK0541)

Mouse VEGF ELISA Kit (Part FPEK0541)
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Price: $645.00
Availability: In Stock
Model: FPEK0541

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Mouse VEGF ELISA Kit (96T)
Highly sensitive kit to mouse VEGF.  Validated for sensitive and selective detection in cell culture medium, serum and plasma.
Lead time approximately 2-3 days.
Highlights
  • Fully configured colorimetric ELISA kit in sandwich fomat.
  • Natural - purified protien provided as standard.
  • Assay range:  15.6pg/ml-1000pg/ml.
  • Validated for cell culture medium, serum and plasma.
  View protocol
 

Kit Contents

Description Quantity
96- well plate precoated with anti-mouse VEGF antibody 1
Lyophilized natural mouse VEGF standard 10ng (1 vial)
Biotinylated anti-mouse VEGF Antibody 130 ul (dilution 1:100)
Sample diluent buffer 30 ml
Antibody diluents buffer 12 ml
ABC diluents buffer 12 ml
TMB color developing agent 10 ml
TMB stop solution 10 ml
 
Background on VEGF
VEGF stands for Vascular permeability factor/vascular endothelial growth factor. It is a cytokine expressed by malignant tumors and has a major role in vasculogenesis, as well as physiological and pathological angiogenesis. VEGF produced by tumor cells strongly stimulates endothelial cell proliferation and angiogenesis. It is also associated with the pathophysiology of several neoplasias. VEGF has been studied for its relation to mediating the development of diabetic retinopathy.
Methods Overview:  Sandwich ELISA
This mouse VEGF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGF has been precoated onto 96-well plates. Standards(from plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. The HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to IL-13 concentration captured on the plate.
   Representative Sandwich ELISA Methods    

  1. Add sample to plate, incubate 90 min, 37oC.
  2. Add detection antibody, incubate 1 hr, 37oC.
  3. Add ABC solution, incubate 30 min 37oC.
  4. Add chromogens, incubate in dark for 25 min.
  5. Add stop solution.  Measure OD at 450nm.

 

 
References

1.     Basu, S.; Nagy, J. A.; Pal, S.; Vasile, E.; Eckelhoefer, I. A.; Bliss, V. S.; Manseau, E. J.; Dasgupta, P. S.; Dvorak, H. F.; Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor. Nature Med. 7: 569-574, 2001.

2.     Poulaki, V.; Mitsiades, C. S.; McMullan, C.; Sykoutri, D.; Fanourakis, G.; Kotoula, V.; Tseleni-Balafouta, S.; Koutras, D. A.; Mitsiades, N. Regulation of vascular endothelial growth factor expression by insulin-like growth factor I in thyroid carcinomas. J. Clin. Endocr. Metab. 88: 5392-5398, 2003.

3.     Awata, T.; Inoue, K.; Kurihara, S.; Ohkubo, T.; Watanabe, M.; Inukai, K.; Inoue, I.; Katayama, S. A common polymorphism in the 5-prime-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes. Diabetes 51: 1635-1639, 2002.

4.     Soker, S.; Takashima, S.; Miao, H. Q.; Neufeld, G.; Klagsbrun, M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor. Cell 92: 735-745, 1998.

5.     Wei, M.-H.; Popescu, N. C.; Lerman, M. I.; Merrill, M. J.; Zimonjic, D. B. Localization of the human vascular endothelial growth factor gene, VEGF, at chromosome 6p12. Hum. Genet. 97: 794-797, 1996. 

 
Safety:  Stop solution contains acid.  Avoid eye contact.  Wear eye protection and gloves.
Storage:  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Shipping.  Overnight delivery


 

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