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InformationADAM10 ELISA KIT (A Disintegrin And Metalloprotease,100 pg-20ng/ml Assay Range: human, mouse or rat; Part hADAM10-ELISA Biotin kw: ADAM10, neurosin, ADAM 10, Desintegrin, Metalloprotease)
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ADAM10 ELISA Kits: A Disintegrin And Metalloprotease ELISA Kits (96T)
Separate kits available for human, mouse and rat ADAM10.
Dynamic assay ranges: 80 pg/ml - 20 ng/ml depending on species. Sensitivity: 20 pg/ml.
Lead Time: Approximately 1.5 weeks
Highlights
Detection Characteristics
Human: Assay range: 100pg/ml - 20ng/ml; Standard Concentration: 24ng/ml.
Mouse: Assay range: 80pg/ml - 20 ng/ml; Standard Concentration: 24ng/ml.
Rat: Assay range: 80pg/ml - 20 ng/ml; Standard Concentration: 24ng/ml.
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a provided biotin-linked detection antibody and provided streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
Sandwich ELISA Methods to ADAM10 
ELISA Assay Steps
Introduction to ADAM10: ADAM10, which is known as a disintegrin and metalloprotease 10, is a cell surface protein enconded by the ADAM10 gene. ADAM10 is a sheddase which cleaves membrane bound proteins at cell surface. After cleavage, the sheddase releases ectodomains with modified location and function. ADAM10 cleaves ephrin, and when this happens, the entire ephrin/eph complex is endocytosed. ADAM10 also has aplha secretase activity which allows processing of amyloid precursor protein. Inhibition of ADAM10 in breast cancer patients shows novel results as ADAM10 shows association with HER2 shedding. The inhibition of ADAM10 decreases HER2 cell lines proliferation.
Gerneral References on ADAM10
1. "Entrez Gene: ADAM10 ADAM metallopeptidase domain 10".
2. Moss ML, Bartsch JW (June 2004). "Therapeutic benefits from targeting of ADAM family members". Biochemistry 43 (23): 7227–35.3. "Entry of ADAM10 endopeptidase (EC-Number 3.4.24.8 )".
4. Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB (October 2005). "Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans". Cell 123 (2): 291–304.
5. Smith KM, Gaultier A, Cousin H, Alfandari D, White JM, DeSimone DW (December 2002). "The cysteine-rich domain regulates ADAM protease function in vivo". The Journal of Cell Biology 159 (5): 893–902.
6. Wolfsberg TG, Primakoff P, Myles DG, White JM (October 1995). "ADAM, a novel family of membrane proteins containing A Disintegrin And Metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions". The Journal of Cell Biology 131 (2): 275–8.
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4°C. 6 months.
Shipping. Overnight domestic delivery recommended with ice pack. Also avaialble for international delivery; please inquire for shipping cost.
kw. adam10 elisa, human, mouse, rat,
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