Protein G Beads (1-2mg/ml)

Protein G binds to most IgG subclasses from human (human IgG1, IgG2, IgG3, IgG4), mouse (mouse IgG1, IgG2a, IgG2b, IgG3) and rat (rat IgG1, IgG2a, IgG2b, IgG2c) species. It can binds strongly to goat (total Ig, IgG1, IgG2), sheep (total Ig, IgG1, IgG2), cow (total Ig, IgG1, IgG2). Protein G can bind slightly with total Ig from rabbit, dog, cat, pig and guinea pig.

This product can be used for 100-200 times. For frequent use, an aliquot can be stored at 4ºC for 1 month with addition of 0.02% sodium azide (NaN3) to the storage buffer. Because this product can purify IgG subclasses from several species of mammals (see above), customers can conjugate the purification products they got with SepharoseTM 4B beads to purify secondary antibody.

  Data Sheet

Specifications

• Matrix: CNBr-activated SepharoseTM 4FF
  
• Beads concentration: 1-2 mg/ml
  
• Coupling conditions of matrix: pH 7-9, 4°C to 25°C, 2-16 h
  
• Binding capacity: 4-7 mg IgG per ml
  
• Bead size range: 45–165 μm
  
• Mean bead size: 90 μm
  
• Bead structure: Highly cross-linked agarose, 4%
  
• Max. flow rate: 4 ml/min/cm2
  
• Recommended flow rate: 1-3 ml/min/cm2