Protein A Beads

• Bead concentration: 1-2 mg/ml
• Matrix: CNBr-activated SepharoseTM 4FF
• Coupling conditions: pH 7-9, 4°C to 25°C, 2-16 h
• Binding capacity: 4-7 mg IgG per ml
• Bead size range: 45–165 μm
• Mean bead size: 90 μm
• Bead structure: Highly cross-linked agarose, 4%
• Max. flow rate: 4 ml/min/cm2
• Recommended flow rate: 1-3 ml/min/cm2
• Stability of the matrix: pH 3-11 (ligand dependent)
• Storage: Store at 4℃ for frequent use, at -20℃ for at least one year

Protein A binds to most human and mouse IgG subclasses (e.g. human IgG1, IgG2, IgG3, IgG4, IgA; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to rat IgG1, IgG2c; goat IgG1, IgG2; sheep IgG1, IgG2; cow IgG1, IgG2; horse IgG (ab), IgG(c). Protein A aslo binds strongly to total IgG from rabbit, dog, cat, pig, guinea pig.

This product can be used for 100-200 times. For frequent use, an aliquot can be stored at 4ºC for 1 month with addition of 0.02% sodium azide (NaN3) to the storage buffer. Because this product can purify IgG subclasses from several species of mammals (see above), customers can conjugate the purification products they got with SepharoseTM 4B beads to purify secondary antibody.