HUMAN sAPP BETA WT ELISA KIT (No. 27732: kw. sAPPbeta, soluble amyloid precursor, appb)

Soluble Amyloid Precursor Protein Beta wild type (sAPPβ-w)ELISA Kit (Human Specificity; 96T)

Sensitive and selective colorimetric ELISA kit to sAPPβ-w with assay range 0.78-50 ng/ml. Highly selective to sAPPβ-w with minimal cross reactivity to sAPPalpha and sAPPβ-sw (see data below). The affinity purified polyclonal capture antibody recognizes the C-terminus of the sAPPbeta cleavage product by Beta-secretase and the monoclonal detection antibody recognizes an epitope on the N-terminus of sAPPbeta.

Fully guaranteed to meet specifications

Specifications:

Complete 96T ELISA kit in sandwich format. Colorimetric detection at 450 nm.
ELISA plate is coated with an affinity purified polyclonal antibody that recognizes the C-terminus sAPPβ-w. The detection antibody is a monoclonal that binds to an epitope on the N-terminus.
The kit detects sAPPβ-w in EDTA-plasma, cerebrospinal fluids, cell culture media or brain tissue extract.
EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
Assay range: 0.78 - 50 ng/ml
Related kit to sAPPalpha is also available: click here

Protocol Overview

Protocol

Epitope Positions

Background on sAPPβ

When beta-secretase cleaves the extracellular domain of amyloid precusor protein (APP) in the first cleavage-processing step towards generating amyloid-beta peptide, the extracellular soluble fragment is known as sAPPbeta. In another cleavage-processing step, gama-secretase embedded in the membrane subsequently cleaves APP within the transmembrane domain and forms amyloid-beta peptide of variable length.¹ This sAPP beta ELISA detects the sAPP beta peptide in cell culture medium and biological fluids, including CSF.

Methods Overview

Equilibrate plate to RT
Add sample to plate, incubate overnight
Add detection antibody, incubate 1 hr
Add color reagents, 30 min
Add stop solution. Measure OD at 450nm

sAPPβ-w ELISA Kit Validation Data

References

Goetzl, E.J., Mustapic, M., Kapogiannis, D., Eitan, E., Lobach, I.V., Goetzl, L., Schwartz, J.B. and Miller, B.L., 2016. Cargo proteins of plasma astrocyte-derived exosomes in Alzheimer’s disease. The FASEB Journal, pp.fj-201600756R.
Selkoe DJ. Normal and abnormal biology of the beta-amyloid precursor protein. Annu Rev Neurosci. 1994;17:489-517.
Citron M, Oltersdorf T, Haass C, McConlogue L, Hung AY, Seubert P, Vigo-Pelfrey C, Lieberburg I, Selkoe DJ. Mutation of the beta-amyloid precursor protein in familial Alzheimer's disease increases beta-protein production. Nature. 1992 Dec 17;360(6405):672-4.
Hsiao K, Chapman P, Nilsen S, Eckman C, Harigaya Y, Younkin S, Yang F, Cole G. Correlative memory deficits, Abeta elevation, and amyloid plaques in transgenic mice. Science. 1996 Oct 4;274(5284):99-102.

Storage: 4^oC, 1 year

Safety: Stop solution contains acid. Avoid contact, wear eye protection.

Shipping: Overnight delivery with styrofoam box and ice packs. International delivery is also available: Please inquire for costs.

kw: sAPPbeta, SOLUBLE AMYLOID PRECURSOR PROTEIN ALPHA, amyloid, amyloid precursor protein, sAPPa, sAPPb, alzheimers, ELISA KIT

Category	Download Link
Protocol Overview	click here
Protocol Manual	click here
MSDS	click here

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