ELISA Kits

ELISA Kits - Fivephoton Biochemicals

Illustrations of kit formats offerred by Fivephoton Biochemicals.  If you do not locate the kit you are interested in, please contact us at customersupport@fivephoton.com for assistance.  We also offer custom ELISA service.

Format I

1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37^oC.  Wash 5 times.
3. Apply chromogens, incubate, then stop reaction.

Format II

1. Equilibrate plate to RT
   
2. Add sample to plate, incubate overnight
   
3. Add detection antibody, incubate 1 hr
   
4. Add color reagents, 30 min
   
5. Add stop solution.  Measure OD at 450nm

Format III

1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37^oC. 
3. Apply chromogen incubate, then stop reaction.

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Examples of targets for ELISA kits offerred by Fivephoton Biochemicals