Human VEGF (Vascular Endothelial Cell Growth Factor) ELISA Kit (96T: 27171)

Human VEGF ELISA Kit

Part No.	27171
Conjugate	HRP
Species	Human
Sample Types	EDTA-plasma,Cell culture supernatant
Assay Range	15.63 ～ 1,000 pg/mL (1st.Incubation for 60 minutes at 37℃)  7.81 ～ 500 pg/mL (1st. Incubation overnight at 4℃)
Primary Reaction	60 minutes at 37℃ or Overnight at 2 - 8℃
Secondary Reaction	30 minutes at 2 - 8℃
Sensitivity	1.01 pg/mL
Specificity	Compound Cross Reactivity Human VEGF 165 100.0% Human VEGF 121 39.7% Human PDGF < 0.1%
Storage	2 - 8 ℃
Package Size 1	96 Well

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VEGF Background

VEGFis a signal protein produced by cells that stimulates the formation of blood vessels.  VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature).

It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus.  VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels.

When VEGF is overexpressed, it can contribute to disease. Solid cancers cannot grow beyond a limited size without an adequate blood supply; cancers that can express VEGF are able to grow and metastasize. Overexpression of VEGF can cause vascular disease in the retina of the eye and other parts of the body. Drugs such as aflibercept, bevacizumab, ranibizumab, and pegaptanib can inhibit VEGF and control or slow those diseases.

 

         Methods Overview

1. Equilibrate plate to RT
   
2. Add sample to plate, incubate overnight
   
3. Add detection antibody, incubate 1 hr
   
4. Add color reagents, 30 min
   
5. Add stop solution.  Measure OD at 450nm

              

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  Protocol Overview

  Protocol

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