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HUMAN AMYLOID BETA TOTAL 1-x ELISA KIT (27729: kw. AB1-x, beta-amyloid 1-x, ABETA

HUMAN AMYLOID BETA TOTAL 1-x ELISA KIT (27729: kw. AB1-x, beta-amyloid 1-x, ABETA
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Price: $679.00
Availability: In Stock
Model: IBL 27729
Manufacturer: IBL Japan

DetailsManuals & DownloadsRelated Products
Amyloid-Beta 1-x ELISA Kit (Human Specificity;  Detects Abeta1-40 & Abeta1-42; 3.55pg/ml Sensitivity, 96T)  

Sensitive and selective colorimetric ELISA kit to ABeta 1-x with assay range 7.81-500 pg/ml, 3.55 pg/ml sensitivity.  Highly selective to amyloid-beta 1-x (see data below).  This ELISA kits employs a monoclonal capture antibody that recognizes the N-terminus and a monoclonal detection antibody that binds near amino acid residue 20 in amyloid-beta:  This ELISA kit detects both Abeta1-40 and Abeta 1-42.

Lead time estimate:  2 weeks
Specifications:
  1. Complete ELISA kit in sandwich format.  Colorimetric detection at 450 nm.
  2. The ELISA plate is coated with an affinity purified monoclonal antibody that recognizes the N-terminus, and a monoclonal detection antibody that binds near amino residue 20.  This kit detects both Abeta40 and Abeta42.
  3. The kit detects amyloid-beta 1-x in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
  4. Incubation times:  Samples with plate;  overnight.  Detection antibody:  1 hr
  5. Assay range:  7.81 - 500 pg/ml.  Sensitivity:  3.55pg/ml
  Protocol Overview

  Protocol

  Epitope Positions

Background on Amyloid-Beta (1-x)

Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by secretases.  Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion.  Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues.  The prevailing isoforms of Amyloid-Beta are Aβ40 and Aβ42.  Aβ40 is the most widespread amyloid-Beta (Aβ) isoform, while Aβ42 is more fibrillogenic and is the principal Abeta peptide associated with Alzheimer’s disease.

Methods Overview
  1. Equilibrate plate to RT
  2. Add sample to plate, incubate overnight
  3. Add detection antibody, incubate 1 hr
  4. Add color reagents, 30 min
  5. Add stop solution.  Measure OD at 450nm

 

Amyloid-beta 1-x ELISA Kit Validation Data


References
  1. Selkoe DJ. Normal and abnormal biology of the -Amyloid precursor protein. Annu. Rev. Neurosci. 17: 489-517, 1994.
  2. Wang R, Sweeney D, Gandy SE, and Sisodia SS. The profile of soluble amyloid protein in cultured cell media. J. Biol. Chem. 271: 31894-31902,1996.
  3. Saido T.C., Iwatsubo T., Mann D.M.A., Shimada H., Ihara Y., and Kawashima S. Dominant and differential deposition of distinct -amyloid peptide species, A N3(pE), in senile plaques. Neuron 14, 457-466, 1995.
Storage:  4oC, 1 year
Safety:  Stop solution contains acid. Avoid contact, wear eye protection.
Shipping:  Overnight delivery with styrofoam box and ice packs.  International delivery is also available:  Please inquire for costs.


 

Category Download Link
Protocol Overview click here
Protocol Manual click here
MSDS click here



 

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