Ferrocene C-6 Phosphoramidite | Ferrocene Phosphoramidite; Electrochemical Oligonucleotide Synthesis (Modification) Reagent | HPT1003

Ferrocene Phosphoramidite is an oligonucleotide synthesis reagent that adds the electromagnetic moiety ferrocene to oligonucleotide chains.  Ferrocene labels have fostered considerable interest in nanotechnology and biosensing applications.

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Reaction scheme of ferrocene phosphoramidite with pentose group of oligonucleotide.

1. Electrochemical detection of biomolecule interaction. ²
2. SNP analysis in medical diagnosis. ⁸
3. Nucleotide hybridization affinity. ¹⁰
4. DNA sequence analysis and genotyping. ¹⁰

References

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2. Drummond TG, Hill MG, and Barton JK. Electrochemical DNA sensors. Nat Biotech 21: 1192, 2003.
3. Ihara T, Maruo Y, Takenaka S, and Takagi M. Ferrocene-Oligonucleotide Conjugates for Electrochemical Probing of DNA. Nucl Acids Res %R 101093/nar/24214273 24: 4273-4280, 1996.
4. Immoos CE, Lee SJ, and Grinstaff MW. Conformationally gated electrochemical gene detection. Chembiochem 5: 1100-1103, 2004a.
5. Immoos CE, Lee SJ, and Grinstaff MW. DNA-PEG-DNA triblock macromolecules for reagentless DNA detection. J Am Chem Soc 126: 10814-10815, 2004b.
6. Navarro AE, Spinelli N, Chaix C, Moustrou C, Mandrand B, and Brisset H. Supported synthesis of ferrocene modified oligonucleotides as new electroactive DNA probes. Bioorg Med Chem Lett 14: 2439-2441, 2004a.
7. Navarro AE, Spinelli N, Moustrou C, Chaix C, Mandrand B, and Brisset H. Automated synthesis of new ferrocenyl-modified oligonucleotides: study of their properties in solution. Nucleic Acids Res 32: 5310-5319, 2004b.
8. Reed MR and Coty WA. eSensor®A Microarray Technology Based on Electrochemical Detection of Nucleic Acids and Its Application to Cystic Fibrosis Carrier Screening. In: Microarrays, 2009, p. 247.
9. Umek RM, Lin SW, Vielmetter J, Terbrueggen RH, Irvine B, Yu CJ, Kayyem JF, Yowanto H, Blackburn GF, Farkas DH, and Chen Y-P. Electronic Detection of Nucleic Acids: A Versatile Platform for Molecular Diagnostics. J Mol Diagn 3: 74-84, 2001.
10. Yu CJ, Wan Y, Yowanto H, Li J, Tao C, James MD, Tan CL, Blackburn GF, and Meade TJ. Electronic detection of single-base mismatches in DNA with ferrocene-modified probes. J Am Chem Soc 123: 11155-11161, 2001.