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InformationRAT/MOUSE AMYLOID-BETA (1-40), 0.28pg/ml Sensitivity, ELISA KIT, IBL 27720: kw. Rat AB40, Rat beta-amyloid 1-40
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Amyloid-Beta 1-40 ELISA Kit (Mouse/Rat Specificity) 96T
Sensitive and selective colorimetric sandwich ELISA kit to Rat ABeta (1-40) with assay range 1.56-100 pg/ml. Highly selective to rat amyloid-beta 1-40 with minimal cross reactivity to human amyloid-beta 1-40 (see data below). The affinity purified monoclonal antibody absorbed on the plate and the affinity purified polyclonal-HRP detection antibody were generated to the N-terminus of rat ABeta 1-40.
A related kit to mouse Abeta 1-42 is also available.
Lead time estimate: Approximately 2 weeks
Specifications:
Specifications:
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Complete ELISA kit in sandwich format. Colorimetric detection at 450 nm.
- ELISA plate is coated with an affinity purified monoclonal antibody that recognizes the N-terminus of rat amyloid-beta 1-40.
- The kit detects rat amyloid-beta 1-40 in EDTA-plasma, serum, cell culture media, or brain tissue extract.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
- Assay range: 1.56 - 100 pg/ml. Sensitivity: 0.28 pg/ml
Protocol Overview
Protocol
Background on Amyloid-Beta (1-40)
Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by certain secretases. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. The prevailing isoforms of Amyloid-Beta are Aβ40 and Aβ42. Aβ40 is the most widespread amyloid-Beta (Aβ) isoform, while Aβ42 is more fibrillogenic and is the principal Abeta peptide associated with Alzheimer’s disease. The affinity purified monoclonal antibody percolated on the ELISA plate and the affiniy purified polyclonal HRP conjugated detection antibody in this kit are selective to rat Aβ40 with insignificant cross-reactivity to human Aβ40. The antibodies have been generated to the N-terminus of rat Aβ40.
Methods Overview
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Equilibrate plate to RT
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Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
Rat/Mouse Amyloid-beta 1-40 ELISA Kit Validation Data
References
- Selkoe DJ. Normal and abnormal biology of the beta-amyloid precursor protein. Annu Rev Neurosci. 1994;17:489-517
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Wang R, Sweeney D, Gandy SE, Sisodia SS. The profile of soluble amyloid beta protein in cultured cell media. Detection and quantification of amyloid beta protein and variants by immunoprecipitation-mass spectrometry. J Biol Chem. 1996 Dec 13;271(50):31894-902.
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Saido TC, Iwatsubo T, Mann DM, Shimada H, Ihara Y, Kawashima S. Dominant and differential deposition of distinct beta-amyloid peptide species, A beta N3(pE), in senile plaques. Neuron. 1995 Feb;14(2):457-66.
Storage: 4oC, 1 year
Safety: Stop solution contains acid. Avoid contact, wear eye protection.
Shipping: Overnight delivery with styrofoam box and ice packs. International delivery is also available: please inquire for international costs.
