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InformationHuman Amyloid-Beta Oligomers (82E1-specific) ELISA Kit, Part No. 27725 IBL: kw. beta-amyloid oligomers, amyloid beta oligomers
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Amyloid-Beta Oligomer (82E1-specific) ELISA Kit (Human Specificity) 96T
Sensitive and selective colorimetric ELISA kit to ABeta Oligomers with assay range 18.98-1215 pmol/L. Highly selective to amyloid-beta oligomers with minimal cross reactivity to amyloid-beta 1-40 peptide (see data below). The affinity purified monoclonal antibody absorbed on the plate and the affinity purified polyclonal-HRP detection antibody are designed to operate as a pair to detect ABeta oligomers.
Lead time estimate: 2 weeks
Specifications:
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Complete ELISA kit in sandwich format. Colorimetric detection at 450 nm.
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ELISA plate is coated with an affinity purified monoclonal antibody, and a provided polyclonal HRP antibody recognize distinct epitopes on amyloid-beta
- The kit detects amyloid-beta oligomers (82E1-specific) in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
- Assay range: 18.98 - 1215 pmol/L
Protocol Overview
Protocol
Background on Amyloid-Beta Oligomers
Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by certain secretases. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. The prevailing isoforms of Amyloid-Beta are Aβ40 and Aβ42. Aβ40 is the most widespread amyloid-Beta (Aβ) isoform, while Aβ42 is more fibrillogenic and is the principal Abeta peptide associated with Alzheimer’s disease. The affinity purified monoclonal antibody percolated on the ELISA plate and the affiniy purified polyclonal HRP conjugated detection antibody in this kit operate as a pair to detect amyloid-beta oligomers.
Methods Overview-
Equilibrate plate to RT
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Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
Amyloid-beta Oligomer (82E1-specific) ELISA Kit Validation Data
References
- Selkoe DJ. Normal and abnormal biology of the -Amyloid precursor protein. Annu. Rev. Neurosci. 17: 489-517, 1994.
- Wang R, Sweeney D, Gandy SE, and Sisodia SS. The profile of soluble amyloid protein in cultured cell media. J. Biol. Chem. 271: 31894-31902, 1996.
- Saido T.C., Iwatsubo T., Mann D.M.A., Shimada H., Ihara Y., and Kawashima S. Dominant and differential deposition of distinct -amyloid peptide species, A N3(pE), in senile plaques. Neuron 14, 457-466, 1995.
- Horikoshi Y., Mori T., Maeda M., Kinoshita N., Sato K., Yamaguchi H., A N-terminal-end specific antibody reduced-amyloid in Alzheimer-model mice. Biochem. Biophys. Res. Commun. 325: 384-387, 2004.
Storage: 4oC, 1 year
Safety: Stop solution contains acid. Avoid contact, wear eye protection.
Shipping: Overnight delivery with styrofoam and ice packs. International delivery is also available: Please inquire for costs.
