Home
Products
Manuals
Log In
Basket
Checkout
RSS
Contacts
Categories
InformationRabbit Amyloid-Beta (1-42) High Sensitivity ELISA Kit. Synonyms: Beta-Amyloid 1-42, AB(1-42), ABeta(1-42. Part No. hAbeta42 (kw. amyloid beta, beta amyloid)
 Click to enlarge |
|
Rabbit Amyloid-Beta (1-42) ELISA Assay Kit. Synonyms: Beta-Amyloid, ABeta, Aß.
Dimentia Disease, Amyloid Synthesis and Degradation Pathway ELISA Product Line
Email customersupport@fivephoton.com for current lead time estimate.
Highlights, Amyloid-Beta (1-42) ELISA Kit
-
Colorimetric detection
-
Rapid Assay: Complete incubations in 2 hrs at RT, or 1 hr at 37oC
-
Suitable for CSF, ISF, serum, plasma, cell and tissue homogenates and lysates
-
Note: Paired rabbit Abeta1-42 (96T) and Abeta1-40 (96T) ELISA kits are also available as a set. View pull-down menu to order the paired set, or ABeta1-42 kits.
-
Abeta1-42 ELISA kits are also available for human, rat, mouse and canine. Inquire for specifications
|
Description
|
Detection Range
|
Stnd Peptide
|
Sensitivity |
|
Rabbit Aß(1-42)
ELISA Kit
|
5 - 240pg/ml
|
360pg/ml
|
3pg/ml |
|
Price (USD)
|
Number of Kits
|
Number of Assays
|
|---|---|---|
|
$635
|
1
|
96T
|
*Must be ordered in sets of 2 kits (2X96T). On ordering online, enter 1 for a set of 2 Kits (Price: $1150.00), enter 2 for a set of 4 kits, etc. Orders can also consist of one rabbit beta-amyloid 1-42 (96T) and one rabbit beta-amyloid 1-40 kit (96T; see related products for description of amyloid-beta 1-40 ELISA kit). Use pull down menu above to order: Cost $1150.
(Use the protocol shipped with the kit for your experiment).
Representative Amyloid-Beta Sandwich ELISA Methods:
Steps of Sandwich ELISA1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add stop solution
6. Colorimetric detection, 450 nm
Sandwich ELISA Method Overview
The provided 96-well ELISA micro-strip plate is pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is then added to block further reaction between HRP and the colorimetric substrates, causing the solution to become yellow. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm.
These ELISA kits provide a standard peptide solution with known concentration to calibrate absorbance readings to concentration.
Background on Protein Targets of the Amyloid ELISA Kits:
Amyloid-Beta (A&η) is generated by sequential cleavage of the amyloid precursor protein (APP) by beta and gamma secretases. Inhibition of secretase activity has been proposed as a therapeutic strategy to limit amyloid-Beta (Aβ) accumulation. Gamma-secretase, which produces the C-terminal end of the Aβ peptide, cleaves within the transmembrane region of APP and generates amyloid-Beta (Aβ) isoforms of 36-43 amino acid residues in length. The dominant isoforms of amyloid-Beta are Aβ40 and Aβ42. Aβ40 is the most common amyloid-Beta (Aβ) isoform, whereas Aβ42 is the more fibrillogenic and the predominant amyloid-beta peptide associated Alzheimer’s disease. Antibodies employed in this Amyloid-Beta ELISA kit are selective for the Aβ42 isoform of amyloid-beta, with insignificant cross-reactivity to the Aβ40 isoform.
Icon Image. Solution structure of the Alzheimer amyloid beta-peptide (1-42) in an apolar microenvironment. DOI:10.2210/pdb1iyt/pdb
Safety, Storage, Shipping
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC. Expires 6 months after manufacture.
Shipping. Overnight domestic delivery recommended with ice pack (cost $57 for 1-2 kits). Also available for international delivery; pleae inquire for shipping cost.
