Leuco-Malachite Green NHS Ester, Part HPT1103

Leuco-Malachite Green NHS Ester, Part HPT1103
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Price: $300.00
Availability: In Stock
Model: HPT1103
Manufacturer: Fivephoton Biochemicals

Available Options:
Leuco-Malachite Green NHS Ester
Amine reactive molecular reporter quenching molecule.  Conjugate to primary amines on peptides, proteins and modified oligonucleotides.


Description:
NAME: Leuco Malachite Green NHS ester
CHEMICAL NAME: 4-[bis-(4-dimethylaminophenyl)-
methyl]-benzoic N-hydroxysuccinimide ester
Formula: C28H29N3O4
MW: 471.550
Purity:  >97%
Appearance: Light green powder
Odor: None
Solubility in Water: Non-Soluble
Solubility:  DMSO, DMF, Acetonitrile
Storage: RT, desiccate.  Ships at ambient temperature

Conjugation Methods:  Use same methods as with Malachie Green NHS Ester, Part 1102 (see protocol below)



Pricing and Quantity.  Use Available Options menu to order.

Quantity
Price (USD)
50mg
$360
100mg
$570
1g
$1680
10g
$7800
5 x 10mg
$360  Reaction Pack










Introduction
Leuco-Malachite Green NHS Ester is a labeling reagent precursor of malachite green.  After leuco-malachite green nhs ester is first linked to a macromolecule through reaction with a primary amino group, and then oxidized with reagents like DDQ, a malachite green modification is generated.  The leuco-malachite green modification can be used as a precursor material for any malachite green derivative that involves a primary amino reaction.  See below for oxidation and conversion methods.

Generation of Malachite Green Modification from Leuco-Malachite Green Modification
Excess DDQ (2,3-dichloro-5,6-dicyano quinone) is dissolved in DMSO or DMF, and added to the solution of leuco malachite green – target conjugate (i.e oligonucleotide previously modified with leuco-malachite green nhs ester).  The leuco-malachite green modification group is immediately oxidized to malachite green, which results in an intense green-blue coloration.  The resulting malachite green – target conjugate can be purified by an appropriate method. 


Reaction Scheme displaying oxidation of leuco-malachite green-nhs ester into malachite green-nhs ester:

         Leuco-Malachite Green-NHS Ester                 Malachite Green-NHS Ester


Safety:  Irritant, avoid contact and inhalation.
Shipping Options:  2 day Fedex shipping (Cost:  $25), and overnight domestic delivery (Cost:  $43). Contact customersupport@fivephoton.com for other delivery options for Leuco-Malachite Green-NHS ester, including international delivery.
Storage:  RT, desiccate. 

 

 


 

Category Download Link
Specification Sheet
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Conjugation Protocol
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COA click here
MSDS click here