Immunofluorescence Labeling Kit (INF-1)

IMMUNOFLUORESCENCE LABELING KIT

Highlights

• For single and multiple immunofluorescent labeling (i.e. labeling with 2-3 fluorescent color tags simultaneously).
  
• The protocol enables detection of antigens in intraellular and extracellular locations.
• Contains saponin for membrane permeabilization.
• Enables detection of cellular organelles such as Golgi, nuclei, mitochondria, endoplasmic reticulum as well as cell surface proteins.
  
• Stock solutions can be thawed-frozen multiple times, or aliquoted and frozen.

Protocol

Immunofluorescence Kit Contents

1. Instruction Manual
2. 10X PBS pH 7.4;  16 ml
3. 10X Quenching Buffer;  25 ml
4. 10X Permeabilization Buffer;  8 ml
5. 10X Intracellular Detection Buffer;  60 ml
6. Forceps set

The immunofluorescence labeling kit employs saponin to permeabilize membranes.  Saponin more effectively preserves membrane structure, enhancing resolution of intracellular organelles in fluorescent microscopy images.  

Along with intracellular antigens, transmembrane and cell surface proteins can also be visualized with immunofluorescence protocols that employ membrane permeabilization since antibodies are accessible to all cellular compartments and locations.

Cellular Regions and Membrane Topology.  This immunofluorescence labeling kit labels proteins in all regions and organelles.  Use a domain selective antibody to exclusively label extracellular or intracellular domains.

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