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InformationHuman Napsin A, 3.91-250ng/mL Assay Range, ELISA Kit, Part No. 27758 IBL: kw. Napsin A, NAPSA
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Napsin A ELISA Kit (Human Specificity)
Specifications:
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Complete 96T ELISA kit in sandwich format. Colorimetric detection at 450 nm.
- ELISA plate is coated with an affinity purified monoclonal antibody that recognizes Napsin A.
- The kit detects human Napsin A in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
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Assay range: 3.91 - 250 ng/ml; 2.92 ng/ml.
Protocol
Methods Overview-
Equilibrate plate to RT
-
Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
Napsin A ELISA Kit Validation Data
References
1. Hirano T, Auer G, Maeda M, Hagiwara Y, Okada S, Ohira T, Okuzawa K, Fujioka K, Franzén B, Hibi N, Seito T, Ebihara Y, Kato H. Human tissue distribution of TA02, which is homologous with a new type of aspartic proteinase, napsin A. Jpn J Cancer Res. 2000 Oct;91(10):1015-21.
2. Hirano T, Gong Y, Yoshida K, Kato Y, Yashima K, Maeda M, Nakagawa A, Fujioka K, Ohira T, Ikeda N, Ebihara Y, Auer G, Kato H. Usefulness of TA02 (napsin A) to distinguish primary lung adenocarcinoma from metastatic lung adenocarcinoma. Lung Cancer. 2003 Aug;41(2):155-62.
3. Dejmek A, Naucler P, Smedjeback A, Kato H, Maeda M, Yashima K, Maeda J, Hirano T. Napsin A (TA02) is a useful alternative to thyroid transcription factor-1 (TTF-1) for the identification of pulmonary adenocarcinoma cells in pleural effusions. Diagn Cytopathol. 2007 Aug;35(8):493-7.
