HUMAN sAPP ALPHA ELISA KIT, sAPPAlpha, SAPPA, Soluble Amyloid Precursor Protein Alpha, IBL 27734: kw. sAPP-alpha, sAPPalpha, soluble amyloid precursor protein

Soluble Amyloid Precursor Protein Alpha (sAPPα)

ELISA Kit (Human Specificity; 96T)

Sensitive and selective colorimetric ELISA kit to sAPPα with assay range 0.78-50 ng/ml. Highly selective to sAPPα with virtually no cross reactivity with human sAPPβ. The capture antibody is a monoclonal and its epitope is positioned at the "alpha" secretase cleavage site at the C-terminus of the sAPPalpha fragement. The detection antibody is also a monoclonal and its epitope is positioned at the N-terminus of sAPPalpha. 96T

Fully guaranteed to meet specifications.

Specifications:

Complete 96T ELISA kit in sandwich ELISA format. Colorimetric detection at 450 nm.
ELISA plate is coated with an affinity purified monoclonal capture antibody positioned at the C-terminus of sAPPalpha. The detection antibody is also a monoclonal and its recognition domain is located at the N-terminus of sAPPalpha.
The kit detects sAPPα in EDTA-plasma, cerebrospinal fluids, serum, and cell culture media.
EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
Assay range: 0.78 - 50 ng/ml; 0.09ng/ml sensitivity
A related kit to sAPPbeta is also available: click here

Protocol Overview

Protocol

Epitope Positions

Background on sAPPα

Cleavage of amyloid precursor protein (APP) by “alpha-secretase” creates the extracellular soluble fragment known as sAPPα. Because the “alpha-secretase” cleavage site is within the amyloid-beta molecule, generation of sAPPα by "alpha-secretase" prevents development of fibrillogenic amyloid-beta. Generation of sAPPalpha may therefore be neuroprotective. sAPPalpha may function in neuronal excitability, synaptic plasticity, synaptogenesis, neurite outgrowth, and cell survival. In addition, sAPPalpha appears to modify potassium channels, N-methyl-D-aspartate receptors, and the transcription factor NF kappa B.

Methods Overview

Equilibrate plate to RT
Add sample to plate, incubate overnight
Add detection antibody, incubate 1 hr
Add color reagents, 30 min
Add stop solution. Measure OD at 450nm

Human sAPPα ELISA Kit Validation Data

References

Selkoe DJ. Normal and abnormal biology of the beta-amyloid precursor protein. Annu Rev Neurosci. 1994;17:489-517.
Citron M, Oltersdorf T, Haass C, McConlogue L, Hung AY, Seubert P, Vigo-Pelfrey C, Lieberburg I, Selkoe DJ. Mutation of the beta-amyloid precursor protein in familial Alzheimer's disease increases beta-protein production. Nature. 1992 Dec 17;360(6405):672-4.
Hsiao K, Chapman P, Nilsen S, Eckman C, Harigaya Y, Younkin S, Yang F, Cole G. Correlative memory deficits, Abeta elevation, and amyloid plaques in transgenic mice. Science. 1996 Oct 4;274(5284):99-102.

Storage: 4^oC, 1 year

Safety: Stop solution contains acid. Avoid contact, wear eye protection.

Shipping: Overnight delivery with styrofoam box and ice packs. International delivery is also available: Please inquire for costs.

kw sAPPALPHA, SOLUBLE AMYLOID PRECURSOR PROTEIN, AMYLOID BETA, sAPPa, ELISA, sAPPbeta, amyloid precursor protein

Category	Download Link
Protocol Overview	click here
Protocol Manual	click here
MSDS	click here

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