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InformationKALLIKREIN-6 (KLK6, NEUROSIN) ELISA KIT (Part No. KLK6-ELISA) kw kallikrein 6, neurosin
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Kallikrein-6 ELISA Kits (KLK6; hK6; Neurosin)
Part No. KLK6-ELISA, 96T)
Lead time: Approx 2 weeks.
Fully guaranteed to meet specifications
Related Kits
* Mouse Specificity Kallikrein-6 ELISA kit with assay range of 200pg/ml to 40ng/ml is also available that uses a monoclonal antibody on the plate (Cost: $635). click here
* Rat Specificity Kallikrein-6 ELISA kit with assay range of 200pg/ml to 40ng/ml is also available that uses a monoclonal antibody on the plate (Cost: $635). click here
KLK6 ELISA Kits. Separate kits are available for human, mouse, rat and canine. Use available options menu to order species specific ELISA kits.
Synonym: Neurosin
Highlights
*(Use the protcol shipped with the kit for your experiment).
Specifications: Kallikrein-6 ELISA Kits
Use Available Options menu to select species specificity of KLK6 ELISA Kits
Background: Kallikrein-6 (KLK-6) is a secretory protein with serine protease activity that is regulated by steroid hormones and implicated in carcinogenesis and amyloid
precursor protein cleavage. Kallikrein-6 also cleaves myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Furthermore, Kallikrein-6 cleaves alpha-synuclein and prevents its polymerization. Kallikrein-6 is detected with gray matter neurons and colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions.
Lead time: approximately 2 weeks.
* An alternate Mouse Specificity Kallikrein-6 ELISA kit with assay range of 200pg/ml to 40ng/ml is also available that uses a monoclonal antibody on the plate (Cost: $635). click here
* An alternate Rat Specificity Kallikrein-6 ELISA kit with assay range of 200pg/ml to 40ng/ml is also available that uses a monoclonal antibody on the plate (Cost: $635). click here
Methods Overview: The included ELISA 96-well micro-strip plate is provided pre-coated with affinity purified rabbit polyclonal antibody selective to antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm. This assay requires approximately 2.5 hrs to complete at RT.
Image Icon: Crystal structure of human Kallikrein-6 with benzamidine inhbitor in active site. Source PDB: DOI:10.2210/pdb1l2e/pdb
Representative Sandwich ELISA Methods 
Steps of Sandwich ELISA
1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add the stop solution
References on Kallikrein-6
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC.
Shipping. Overnight domestic delivery recommended with styrofoam box/ice pack (cost: $52 for 2 kit set). Also available for international delivery; please inquire for shipping cost.
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