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InformationBACE1 ELISA KIT, 5pg/ml Sensitivity, Highly Sensitive, Rapid Protocol (Human, rat or mouse, Beta-Secretase 1, B-Secretase, Beta-Site APP Cleaving Enzyme 1; Part BACE1-ELISA)
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High Sensitivity BACE1 ELISA Kits (96T)
Email customersupport@fivephoton.com for current lead time estimate.
(Beta-Secretase 1, Beta-Site APP Cleaving Enzyme)
Human, rat, mouse, canine, rabbit kits available
Fully guaranteed to meet specifications
Part No. h, r, m, cn, rb, BACE1-ELISA
Sensitive 5-200 pg/ml detection range for human, 96T. $599. Rapid protocol sandwich ELISA kit. Separate kits are available for human, mouse, rat, canine and rabbit Beta-Secretase 1
Highlights
Specifications BACE1 ELISA Kits
Use Available Options menu to select species specificity of the BACE1 ELISA Kits
Background: β-Secretase (BACE1) is an aspartyl protease involved in the cleavage of amyloid precursor protein (APP). BACE1 is a transmembrane protein with two active site aspartate residues in its extracellular protein domain. BACE1 may operate functionally as a dimer. Generation of amyloid-beta peptide requires two sequential cleavages of amyloid precursor protein (APP). Extracellular cleavage of APP by BACE1 creates a soluble extracellular fragment and a cell membrane-bound fragment. Cleavage of a membrane bound fragment by γ-secretase releases the intracellular domain of APP and yields amyloid-beta peptide. Levels of BACE1enzymatic activity appear to be elevated in age related, late-onset Alzheimer's potentially suggesting a gene regulatory mechanism.
Image Icon. Crystal structure of human BACE1 at pH 7.0. Source: PDB: DOI:10.2210/pdb2zhv/pdb
Methods Overview: The included 96-well ELISA micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added which upon conversion by HRP become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution to yellow. An absorbance plate reader is used to quantitate the colorimetric reaction at 450nm. The assay requires approximately 2.5 hrs to complete at RT, including incubations with plate and HRP antibody, washes, and color reaction.
Image Icon: Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40). PDB: DOI:10.2210/pdb2g47/pdb
Representative Sandwich ELISA Methods 
Steps of Example Sandwich ELISA
1. Equilibrate plate to ambient temperature
2. Add sample, HRP detection Ab, incubate 30 min at 37oC
3. Wash
4. Add the color reagents
5. Add the stop solution
Protocol
Product Citations For Part BACE1-ELISA
General References on BACE1
For research only. Not for diagnostics of disease in human or animals.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC, 6 months. Standard, strip plate sections of the ELISA plate, and other solutions, can be stored longer term at -20oC.
Shipping. Overnight domestic delivery recommended. Also available for international delivery; please inquire for shipping cost.
KW: BACE1, mouse, rat, rabbit, canine, human beta-secretase, bACE, beta-site APP cleaving enzyme ELISA Kit, alzheimer's, APP cleavage.
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