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InformationPresenilin 2 ELISA Kit (PSEN2, PS-2, PS2; Part No. PS2-ELISA) ELISA Kit
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Presenilin 2 ELISA Kits (PSEN2, PS-2, PS2; Part PS2-ELISA)
High Sensitivity
Separate ELISA kits are available for human, mouse, rat, rabbit and canine Presenilin 2. Use available options menu to order species specific ELISA kits.
Lead time: approximately 2 weeks.
 View General ProtocolSpecifications of Presenilin-2 ELISA Kits
*Use Available Options menu to select species
Overview: Presenilins are a family of related multi-pass transmembrane proteins that function as a part of the gamma-secretase protease complex. Vertebrates have two presenilin genes, called PSEN1 (located on chromosome 14 in humans) that encode Presenilin 1 (PS-1) and PSEN2 (on chromosome 1 in humans) that codes for presenilin 2 (PS-2). Both genes show conservation between species, with little difference between rat and human presenilins.
Presenilins undergo cleavage in an alpha helical region of one of the cytoplasmic loops to produce a larger N-terminal and a smaller C-terminal fragment, which together form part of the functional protein. Cleavage of presenilin 1 can be prevented by a mutation which causes the loss of exon 9, and results in loss of function. Presenilins play a key role in the modulation of intracellular Ca2+ involved in presynaptic neurotransmitter release and long-term potentiation induction.
Dominant mutations in the genes that encode presenilin proteins are the most common cause of familial early-onset Alzheimer's disease.
Use available options menu to select species.
Methods Overview: The provided ELISA 96-well micro-strip plate is coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm. This assay requires approximately 2.5 hrs to complete at RT.
*(Use the protcol shipped with the kit for your experiment).
Icon Image: Cleavage pathway of amyloid precursor protein into beta-amyloid.
Representative Sandwich ELISA Methods 
Steps of Sandwich ELISA
1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add the stop solution
References on Presenilin 2
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC.
Shipping. Overnight domestic delivery recommended; flat rate continental US costs: $52. Also available for international delivery; please inquire for shipping cost.
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