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BETA-SYNUCLEIN (SNCB) ELISA Kit, 110pg/ml Sensitivity, (Human, rat, or mouse; B synuclein, beta synuclein; Part No BSyn-ELISA; 96T)

BETA-SYNUCLEIN (SNCB) ELISA Kit, 110pg/ml Sensitivity, (Human, rat, or mouse; B synuclein, beta synuclein; Part No BSyn-ELISA; 96T)
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Price: $640.00
Availability: In Stock
Model: BSyn-ELISA

Available Options:
Select Species:
DetailsManuals & DownloadsRelated Products
SNCB Beta-Synuclein ELISA Kits 
Sandwich ELISA format (96T)
Fully guaranteed to meet specifications

Highlights
  • Dynamic assay range 200pg/ml - 60ng/ml for human SNCB.
  • Separate kits are available for human, mouse and rat beta-synuclein. 
  • Highly selective to beta-syncuclein (SNCB) with insignificant cross-reactivity to other synucleins.
  • Sandwich ELISA format providing convenience and high sensitivity. 
  • Strip plate format allowing separate assay and storage of sections.
  • Rapid protocol:  Only one incubation with antibodies.  Total protocol time approximately 1.5 hrs.
  • Colormetric detection at 450nm using a standard absorbance plate reader.

Price (USD)
Number of Kits
Number of Assays
$640
1
96T







  Specifications (All kits are 96T)
Species
 Part
Standard Peptide
Assay Range
 Sensitivity
Human
 hBSyn-ELISA
80 ng/ml
200pg/ml - 60ng/ml
 241 pg/ml
Mouse
 mBSyn-ELISA
64 ng/ml
200pg/ml - 60ng/ml
 176 pg/ml
Rat
 rBSyn-ELISA
64 ng/ml
200pg/ml - 60ng/ml
 188 pg/ml
                               








 
*Use Available Options Menu to select species
Beta-Synuclein (SNCB) is a member of the synucelin protein family.  Beta-synuclein is highly expressed in the substantia nigra, a region of neuronal degeneration in Parkinson's disease patients.  Beta-synuclein may inhibit alpha-synuclein aggregation associated with Parkinson's disease, potentially providing protection from the toxicity associated with alpha-synuclein aggregation.  This ELISA kit is highly selective to beta-synuclein with insignificant cross-reactivity to gamma and alpha-synuclein.
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody highly selective to SNCB.  Samples are pre-mixed with a biotin-linked polyclonal detection antibody specific to beta-synuclein and streptavidin-HRP;  the mixture is then added to the plate and incubated for 60 min.  The plate is washed after the incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction.  The solution is measured at 450nm.  


   Sandwich ELISA Methods                                                                                                            

Rapid Protocol ELISA Assay Steps
 
  1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Afterwards, wash 5 times.
  3. Apply chromogens, incubate, then stop the reaction.
 



Kit Contents
  1. 8 x 12 well removable section strip plate coated with anti-SNCB monoclonal antibody
  2. Anti-SNCB polyclonal detection antibody conjugated with biotin
  3. Steptavidin-HRP
  4. Protein Standard
  5. Standard diluent
  6. Concentrated wash solution
  7. Chromogen solutions A and B
  8. Stop solution
  9. Plate sealers
Protocol

Sorry, the PDF couldn't be displayed :(



Product Citation:

Wilaiwan Sriwimol and Pornprot Limprasert, “Significant Changes in Plasma Alpha-Synuclein and Beta-Synuclein Levels in Male Children with Autism Spectrum Disorder,” BioMed Research International, vol. 2018, Article ID 4503871, 7 pages, 2018. doi:10.1155/2018/4503871  Link to article

References on Beta-Synuclein (SNCB)

  1. Giasson, B. I., Duda, J. E., Forman, M. S., Lee, V. M., & Trojanowski, J. Q. (2001). Prominent perikaryal expression of alpha- and beta-synuclein in neurons of dorsal root ganglion and in medullary neurons. Experimental Neurology, 172(2), 354-362.
  2. Lincoln, S., Crook, R., Chartier-Harlin, M. C., Gwinn-Hardy, K., Baker, M., Mouroux, V., Richard, F., et al. (1999). No pathogenic mutations in the beta-synuclein gene in Parkinson’s disease. Neuroscience Letters, 269(2), 107-109.
  3. Ohtake, H., Limprasert, P., Fan, Y., Onodera, O., Kakita, A., Takahashi, H., Bonner, L. T., et al. (2004). Beta-synuclein gene alterations in dementia with Lewy bodies. Neurology.
  4. Rivers, R. C., Kumita, J. R., Tartaglia, G. G., Dedmon, M. M., Pawar, A., Vendruscolo, M., Dobson, C. M., et al. (2008). Molecular determinants of the aggregation behavior of alpha- and beta-synuclein. Protein Science, 17(5), 887-898. COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEP
Safety, Storage, Shipping, Beta-Synuclein ELISA Kit
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4oC.  Expires 1 year after manufacture.
Shipping. Lead time 2 weeks.  Overnight domestic delivery recommended.  Also available for international delivery.  Please inquire for international delivery rates.
kw.  sncb, beta-synuclein, elisa kit, synuclein, parkinson's


 

Category Download Link
Protocol Manual click here
MSDS click here



 

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