Mouse Cyclophilin B ELISA Kit (CYPB, PPIB, Peptidylprolyl Isomerase B) Part No. mCYPB-ELISA

Mouse Cyclophilin B (CYPB, PPIB) ELISA Kit, 96T

Highlights of the Cyclophilin B (CyPB) ELISA Kit

• Fully pre-configured sandwich ELISA format providing convenience, high selectivity and sensitivity.
• Affinity purified polyclonal capture antibody on plate.  Separate HRP conjugated detection antibody.
  
• Strip plate configuration enabling separate use and storage of sections of the ELISA plate.
• Separate kits are offered for human (h), rat (r) and mouse (m).
  
• Incubation Time.  First incubation:  1 hr at ambient temperature;  Second incubation with anti-p65 HRP;  1 hr at ambient temperature.  Alternatively, 30 min incubations can be performed at 37degrees C for both steps.
  
• Detection Method:  Colorimetric at 450 nm.

Synonyms:  PPIB; PPI-B; CYP-S1; CyP-B; SCYLP; Peptidylprolyl Isomerase B; Peptidyl-Prolyl Cis-Trans Isomerase B.  Separate Cyclophilin B (CYPB) kits are available for human, rat and mouse (see Related Products tab). 

Lead time approximately 2 weeks.

View Protocol

Overview:  Cyclophilin B (CyPB) is a cyclosporine-binding protein located mainly within the endoplasmic reticulum.  It has also been detected in the nucleus where it may maintain a gene regulatory role.  Cyclophilin B has a signal sequence and is therefore associated with the secretory pathway and secreted into biological fluids. 

Given that Cyclophilin B is a soluble protein in the secretory pathway, the primary fractions to detect and assay are in biological fluids, such as serum and plasma, cell culture supernatant, and cell and tissue lysates.  Use the isolation methods suggested in the protocol manual for biological fluids and cell culture supernatant.  To assay in cell or tissue lysates, use a non-denaturing lysis buffer to solubilize membranes and suspend intracellular Cyclophilin B into the lysis buffer supernatant.  The  protocol associated with the FIVEphoton Biochemicals ELISA Lysis and Protein Extraction Buffer (Part No. ELSP-1) provides detailed instructions to isolate intracellular proteins by cell lysis. 

Protocol Synopsis of Sandwich Cyclophilin B (CyPB) ELISA