Patch Clamp Pipettes | Pre-Pulled Glass Micropipettes | FIVEphoton Biochemicals

Pre-Pulled Glass Micropipettes

Patch Clamp Pipettes

Pre-Pulled Glass Micropipettes: Part No. PCPs-1A

Cost: $185 for 24 pipettes. See price table below for other quantities.

2-3 day lead time

Highlights:

Precise fabrication to specifications
Packaged for stable shipping and storage
Multiple applications including microfluidics and patch clamping

Parameters, Part No PSPs-1A:

Pre-Pulled Micropipettes

Glass: Thin wall borosilicate, with capillary (also available w/o capillary for bioengineering applications).
Total length of pipette 5.5 cm
Tip length 2-3 µm
Outer diameter at tip (OD) 1.5 µm
Wall thickness at base 0.17mm
Outer diameter at base (OD): 1.5 mm
Pipette resistance (in standard saline) 1-3 MegaOhm
Pipettes pulled on highly stable Sutter Instruments P-97 puller
View Image tab revealing internal capillary

Patch Pipettes are freshly manufactured and individually secured in packaging immediately prior to shipping. On online orders, in the comments box, write with or without capillary. For alternate pre-configured pipettes, see Microfluidics Gass Micropipettes, Part MGM-1.

View picture of pre-pulled micropipettes in images and data tab

Quantity	Cost (USD)
12	$115.00
24	$185.00
100	$695.00

Select pre-configured pipettes on available options menu.

Custom Pre-Pulled Micropipettes: Part No. PCPs-1B

Stable for shipping and storage

Cost: $210 for 20 pipettes plus $50 first time setup charge. Lead time: 7 days

To order custom pipettes, contact customersupport@fivephoton.com or Tel: 800-462-4507 for quote

Applications: IVF, microinjection, focal saline application, cell collecting, voltage/current-clamp, single channel recordings, single cell pharmacology, microfluidics, microapplication on chip, micropipette applications.

Parameters:

Glass: Customer request
With or without internal capillary
Length: Customer request
Outer diameter (OD): Customer request
Wall thickness: Customer request
Tip outer diameter: Customer request
Tip inner diameter: Customer request
Tip is fire-polished on micro forge by Customer request
Pipette resistance (in standard saline): Customer request
Sterilized with heat, or UV by Customer request

Pipette-Cell Image: Adult rat trigeminal mesencephalic neurons.

Also view Microfluidics Glass Micropipettes (Part No. MGM-1) for alternate configurations and applications.

Representative Electrophysiology Recordings

3µM BRUS-3145 200nM omega-AgaIVA+5microM BRUS-3145

Figure Legends:

Upper panel demonstrates stability of Ca⁺⁺ channels and potentiation effect of experimental substance BRUS-3145 recorded with patch pipettes produced by FIVEphoton Biochemicals isolated cortex neurons of rat. w-Aga-IVA does not block the BRUS-induced potentiation of the Ca⁺⁺ channels. The cortex neuron which demonstrated the potentiation effect under the addition of 3mM BRUS-3145 was then incubated in 200nM w-Aga-IVA. Left panel: IV relationships measured in control (open circles) and after application of 3mM BRUS-3145 (filled circles). Right panel: IV relationships measured after 3 min incubation in w-Aga-IVA (open triangles) and after subsequent application of 3mM BRUS-3145 (filled triangles). The recovery (squares) was obtained within 1 min in both cases.

Data – courtesy of Light Biologicals, Inc

Storage: RT

Shipping recommendation: Domestic 2-day Fedex ($18 flat rate) or domestic overnight delivery ($43 flat rate). Also available for international shipping: please inquire with customersupport@fivephoton.com for international shipping rate.

kw. pre-pulled glass micropipettes, borosilicate micropipettes, micropipettes.

Document	Download Link
Specification Sheet - Part PCPs-1A	click here
Specification Sheet - Part MGM-1	click here

Category	Download Link
Protocol Manual	click here
MSDS	click here

Patch Clamp Pipettes | Pre-Pulled Glass Micropipettes | FIVEphoton Biochemicals | PCPs-1; PCPs-1A; PCPs-1B

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FIVEphoton Fluorescent Membrane Potential Assay Kit - High Throughput Format (Part. No. mpHTS-1) mpHTS-Kit $1,420.00	FIVEphoton Membrane Potential Assay Kit \| FIVEphoton Biochemicals \| MPF-Kit MPF-Kit $250.00	Membrane Potential Fluorescent Dyes (DiSBAC1(3), DisBAC2(3), DiBAC1(3)) \| FIVEphoton Biochemicals \| Disbac1-4 DiSBAC 1-4 $190.00	Patch Clamp Solutions (Intracellular and Extracellular Buffers \| FIVEphoton Biochemicals \| PctS-100I and Pcts-100E) PctS-100 $65.00
Chloride Channel Patch Clamp Recording Solutions (Part Nos. ES0Cl and ISCI) Cl-Solns $100.00	Microfluidics Glass Micropipettes (Pre-Pulled Micropipettes; Part No. MGM-1) MGM-1 $210.00

Tel: 800.462.4507 Fax: 888.694.2610
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Patch Clamp Pipettes | Pre-Pulled Glass Micropipettes | FIVEphoton Biochemicals | PCPs-1; PCPs-1A; PCPs-1B