Patch Clamp Pipettes | Pre-Pulled Glass Micropipettes |Pre-Pulled Pipettes

Pre-Pulled Glass Micropipettes

Patch Clamp Pipettes

Pre-Pulled Glass Micropipettes: Part No. PCPs-1A

Highlights:

Pipettes are freshly fabricated for each order
Packaged for stable shipping and storage
Multiple applications including microfluidics, printing and patch clamping
Made in the United States
Pre-configured and custom configured micropipettes are available
Not fire polished
Multiple additional configurations are available at part MGM-1 (click here)

Standard Pre-Pulled Glass Micropipettes

Pre-Pulled Micropipettes - Standard Configuration. $419 for 30 pipettes, $1129 for 100 pipettes

Parameters, Part No PCPs-1A:

Glass: thin wall borosilicate, with or without internal filament *
Total pipette length: Approx 5 cm
Outer diameter at tip (ODtip): 1.5 ± 0.3 um
Inner diameter at tip (IDtip): 1.2 ± 0.24 um
Taper length: 1-5 mm
Wall thickness at base: 0.17 mm
Outer diameter at base (ODbase): 1 or 1.5 mm depending on request
Pipettes pulled on highly stable Sutter Instruments P-97 puller
Not fire polished

Specification Sheet

Handling Instructions

Standard Pre-Pulled Glass Micropipettes

Quantity	Part No.	Cost (USD)
30 pipettes	PCPs-1A(30f) with filament	$489.00
30 pipettes	PCPs-1A(30wf) without filament	$489.00
100 pipettes	PCPs-1A(100f) with filament	$1229.00
100 pipettes	PCPs-1A(100wf) without filament	$1229.00

Select pipettes on available options menu.

For additional configurations for pre-pulled glass micropipettes, see Part MGM-1 (click here).

* For online orders, please write with or without filament in the comments box

Custom Pre-Pulled Micropipettes: Part No. PCPs-1B

Cost: $419 - $490 for 30 pipettes, depending on configurations. Lead time: 14 days

To inquire and/or order custom pipettes, contact customersupport@fivephoton.com or Tel: 1.858.395.4026.

Applications: IVF, microinjection, focal saline application, cell collecting, voltage/current-clamp, single channel recordings, single cell pharmacology, microfluidics, microapplication on chip, molecular printing, micropipette applications.

Parameters:

Glass: Customer request
With or without internal capillary
Length: Customer request
Outer diameter (OD): Customer request
Wall thickness: Customer request
Tip outer diameter: Customer request
Tip inner diameter: Customer request
Pipette resistance (in standard saline): Customer request

Pipette-Cell Image: Adult rat trigeminal mesencephalic neurons.

Product Reference

Bonvini, Sara J., et al. "Transient receptor potential cation channel, subfamily V, member 4 and airway sensory afferent activation: Role of adenosine triphosphate." Journal of Allergy and Clinical Immunology (2016).

Link to article

Representative Electrophysiology Recordings

3µM BRUS-3145 200nM omega-AgaIVA+5microM BRUS-3145

Figure Legends:

Upper panel demonstrates stability of Ca⁺⁺ channels and potentiation effect of experimental substance BRUS-3145 recorded with patch pipettes produced by FIVEphoton Biochemicals isolated cortex neurons of rat. w-Aga-IVAdoes not block the BRUS-induced potentiation of the Ca⁺⁺ channels. The cortex neuron which demonstrated the potentiation effect under the addition of 3mM BRUS-3145 was then incubated in 200nM w-Aga-IVA. Left panel: IVrelationships measured in control (open circles) and after application of 3mM BRUS-3145 (filled circles). Right panel: IV relationships measured after 3 min incubation in w-Aga-IVA (open triangles) and after subsequent application of 3mM BRUS-3145 (filled triangles). The recovery (squares) was obtained within 1 min in both cases.

Data – courtesy of Light Biologicals, Inc

Storage: Room temperature.

Packing is stable for international delivery to South America, Canada, Europe, Middle East and Asia: Please inquire with customersupport@fivephoton.com for international shipping rate.

kw. pre-pulled glass micropipettes, borosilicate micropipettes, micropipettes, pre-pulled pipettes, pre-pulled capillaries, glass needles.

Terms and Warranty

Pipettes are manufactured to substantially conform with the descriptions on this webpage. However, the customer should note that variability exists in the manufacturing process, estimated from 10-20%, with respect to tip diameter, taper length, and tip angle within and between lots. The customer should communicate prior to ordering and manufacture any specialized requirements with respect to these parameters, including restriction to variability within and between lots. Specific applicability, fitness and merchantability are not warranted. Warranty compliance is met by replacement, full or partial refund on Fivephoton Biochemicals discretion.

Document	Download Link
Handling Instructions	click here
Specification Sheet - Part PCPs-1A	click here
Specification Sheet - Part MGM-1	click here
Printable Product Sheet	click here

Category	Download Link
Protocol Manual	click here
MSDS	click here

Patch Clamp Pipettes | Pre-Pulled Glass Micropipettes |Pre-Pulled Pipettes | PCPs-1; PCPs-1A; PCPs-1B

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FIVEPHOTON FLUORESCENT MEMBRANE POTENTIAL ASSAY KIT - High Throughput Format (Part. No. mpHTS-Kit) mpHTS-Kit $390.00	FIVEPHOTON MEMBRANE POTENTIAL ION CHANNEL ASSAY KIT \| Fluorescent Voltage Sensitive Dye Kit (kw. Voltage-sensitive probes: Part MPF-Kit) MPF-Kit $390.00	Membrane Potential Assay Fluorescent Dyes (Syn: Voltage sensitive dyes, DiSBAC1(3), DisBAC2(3), DiBAC1(3)) \| FIVEphoton Biochemicals \| Disbac1-4 DiSBAC 1-4 $190.00	Patch Clamp Solutions (Intracellular and Extracellular Buffers \| FIVEphoton Biochemicals \| PctS-100I and Pcts-100E) PctS-100 $85.00
Chloride Channel Patch Clamp Recording Solutions (Part Nos. ES0Cl and ISCI) Cl-Solns $100.00	Glass Micropipettes - Pre-Pulled Microcapillaries for Microfluidic Applications (Pre-pulled Micropipettes; Part No. MGM-1 x) MGM-1x (30 pipettes) $489.00	Perfusion Dye Tracer Kit (Diffusion Tracer, Part Nos. PDTK-1 and PDTK-2) PDTK $129.00

Tel: 1.858.395.4026; or 1.800.462.4507
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Patch Clamp Pipettes | Pre-Pulled Glass Micropipettes |Pre-Pulled Pipettes | PCPs-1; PCPs-1A; PCPs-1B