CHAPS Immunoprecipitation (IP) and Lysis Buffer For Tissues and Cells | CHAPS Buffer | Part No. CIB-1

CHAPS Immunoprecipitation (IP) and Lysis Buffer  For Tissues and Cells | CHAPS Buffer | Part No. CIB-1
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Price: $72.00
Availability: In Stock
Model: CIB-1
Manufacturer: Fivephoton Biochemicals

Available Options:
CHAPS Buffer pH 8.0:
CHAPS Buffer pH 7.4:
CHAPS Buffer pH 6.0:
Immunoprecipitation (IP) and Cell Lysis Buffer with CHAPS Detergent

Applicable for immunoprecipitation with tissue and culture cell samples

CHAPS immunoprecipitation and lysis buffer with 0.5% CHAPS, HEPES, NaCl, 1X Solution.  Preserves native protein conformation
Available Volumes
60 ml
120 ml

Available Formulations
  Part No
 pH 8.0
 pH 7.4
 pH 6.0

*All pH's are offered at the same price. 

Additional Options
CaCl2 and EDTA Options*
a)  Addition of 1 mM EDTA
b)  Addition of 1 mM CaCl2
*Addition of CaCl2 or EDTA does not affect price.

The addition of calcium chloride to the CHAPS reagent preserves calcium dependent protein-protein interaction, whereas the addition of EDTA abrogates the interaction. 

Highlights: CHAPS Buffer 
  • Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis.
  • Can be used with tissues (see product citation 1 below) as well as culture cells. 
  • Effective as a stand alone reagent for cell lysis, protein extraction and subsequent Western blot in addition to immunoprecipitation.
  • Contains a non-amine buffer (HEPES) enabling cross-linking with NHS-ester derivatives. 
    Ingredients:  0.5% CHAPS, HEPES, NaCl
  • Compatible with Bradford, Lowry and BCA protein assays.
  • 60 ml volume:  Suitable for two hundred 10 cm culture plates, or six hundred wells in 6-well dishes.  120 ml volume for four hundred 10 cm culture plates or 1200 wells in 6-well dishes.
  • Storage:  4oC.  Ships at ambient temperature.

Representative data with CHAPS Immunoprecipitation and Cell Lysis Reagent (pH 8.0)

Figure 1 
Co-immunoprecipitation (Co-IP) of a trafficking protein receptor (COPII) with a cation channel (TRPC-6).  HEK293 cells were co-transfected with plasmid DNA encoding TPRC-6 and a HA epitope tagged component of COPII (sec24).  Cell lysis and immunoprecipitation were performed in CHAPS Buffer as summarized in the product manual.  Western blotting (WB) was performed with the antibodies indicated in the figure.

                              IP:  Anti-HA COPII                      IP:  Anti-HA COPII
                                WB: Anti-TRPC6                        WB:  COPII

Figure 2 
Co-immunoprecipitation (Co-IP) of a calcium binding protein with a multisubunit target protein 1 employing the FIVEphoton CHAPS immunoprecipitation reagent.  A larger amount of target protein 1 associates with the calcium binding protein when expressed alone, as compared to co-expression (and presumed assembly) with protein 2.  Protein 1 reveals higher molecular weight forms due to post-translational modifications.

Outline of immunoprecipitation methodology with CHAPS Buffer (see protocol for details)

©Fivephoton Biochemicals 2010

Product Citations

1.   Drum, Benjamin ML, et al. "Oxidative stress decreases microtubule growth and stability in ventricular myocytes." Journal of molecular and cellular cardiology 93 (2016): 32-43.  Link to article

2.    Villa‐Diaz, Luis G., et al. "Inhibition of Focal Adhesion Kinase Signaling by Integrin α6β1 Supports Human Pluripotent Stem Cell Self‐Renewal." Stem Cells (2016).  Link to article
3.    Kurland, D. B., Gerzanich, V., Karimy, J. K., Woo, S. K., Vennekens, R., Freichel, M., ... & Simard, J. M. (2016). The Sur1-Trpm4 channel regulates NOS2 transcription in TLR4-activated microglia. Journal of Neuroinflammation, 13(1).  Link to article

4.     Qian, X., Kim, J.K., Tong, W., Villa‐Diaz, L.G. and Krebsbach, P.H., 2015. DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover. STEM CELLSLink to article

5.  Zhang G, Brady J, Liang W-C, Wu Y, Henkemeyer M, Yan M. EphB4 forward signalling regulates lymphatic valve development. Nature Communications. 2015;6:6625. doi:10.1038/ncomms7625.  Link to article

6.  Shuchong Pan et. al.  2014.  Cell Surface Protein Disulfide Isomerase  Regulates Natriuretic Peptide Generation of Cyclic Guanosine Monophosphate.  Plos One.  DOI: 10.1371/journal.pone.0112986.  Link to article


Trial Samples:  Free 4 ml trial samples are available for Biotech/Pharma customers:  Please forward your request to

General References on CHAPS Immunoprecipitation and Cell Lysis 
  1. Darby PJ, Kwan CY, Daniel EE. 2000. Caveolae from canine airway smooth muscle contain the necessary components for a role in Ca(2+) handling. Am J Physiol Lung Cell Mol Physiol 279: L1226-35.
  2. Farmery MR, Allen S, Allen AJ, Bulleid NJ. 2000. The role of ERp57 in disulfide bond formation during the assembly of major histocompatibility complex class I in a synchronized semipermeabilized cell translation system. J Biol Chem 275: 14933-8.
  3. Ora A, Helenius A. 1995. Calnexin fails to associate with substrate proteins in glucosidase-deficient cell lines. J Biol Chem 270: 26060-2.
  4. Pieren M, Gali C, et. al. 2005. The use of calnexin and calreticulin in cellular and viral glycoproteins.  J Biol Chem 280:  28265-71.  
  5. Zhu X, Peng J, Chen D, Liu X, Ye L, et al. 2005. Calnexin and ERp57 facilitate the assembly of the neonatal Fc.  Journal of Immunology 175: 967-76.

Accessory  Products
In addition to direct orders, this item can be purchased on and shipped to the customer from Fivephoton Biochemicals: item no. NC0221482, NC0982453.  CHAPS buffer (CIB-1) is also available from distributors of Fivephoton Biochemicals.

Safety:  Irritant.  Avoid skin and eye contact and ingestion.
Storage:  4oC.
Shipping:   Ambient temperature shipping.  Domestic Fedex Ground ($8:00 flat rate),  2-day (flat rate $24) or Fedex overnight delivery (flat rate $38).  International delivery is also available.  Please inquire for international shipping rates. 




Category Download Link
Protocol Manual click here
MSDS click here


CHAPS Immunoprecipitation (IP) and Lysis Buffer  For Tissues and Cells | CHAPS Buffer | Part No. CIB-1
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