PROTEASE INHIBITOR COCKTAIL BROAD SPECTRUM (100X; PI-1)

• Broad spectrum protease inhibitor cocktail.
• Inhibitor of serine and cysteine proteases, and aminopeptidases.
• Provided as lyophilized solid.  Suspend with 1ml dH20.  Add aliquot of protease inhibitor cocktail directly into cell lysates.
• Store lyophilized and solubilized material at -20^oC.  Lyophilized material ships at ambient temperature.
  

References on protease inhibition

1. Altan H, Bozkurt AK, Arslan C, Ustundag N, Konukoglu D, and Koksal C. Serine protease inhibitor aprotinin ameliorates renal injury in a rat model of ischemia-perfusion injury. Transplant Proc 41: 1512-1516, 2009.
2. Arai T, Ikarashi Y, Okamoto K, Kuribara H, and Maruyama Y. Memory disturbance and hippocampal degeneration induced by continuous intraventricular infusion of a protease inhibitor, leupeptin. Brain Res 754: 157-162, 1997.
3. Christoffers KH, Khokhar A, Chaturvedi K, and Howells RD. Inhibition of mu and delta opioid receptor ligand binding by the peptide aldehyde protease inhibitor, leupeptin. Regul Pept 105: 9-14, 2002.
4. Citron M, Diehl TS, Capell A, Haass C, Teplow DB, and Selkoe DJ. Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF. Neuron 17: 171-179, 1996.
5. Hussein EM, Dawoud HA, Salem AM, and Atwa MM. Antiparasitic activity of cystine protease inhibitor E-64 against Giardia lamblia excystation in vitro and in vivo. J Egypt Soc Parasitol 39: 111-119, 2009.
6. Jose J and Zangen D. Autodisplay of the protease inhibitor aprotinin in Escherichia coli. Biochem Biophys Res Commun 333: 1218-1226, 2005.
7. Nakabo Y and Pabst MJ. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. J Leukoc Biol 60: 328-336, 1996.
8. Rudzinski T, Mussur M, Gwiazda Z, and Mussur M. Protease inhibitor leupeptin attenuates myocardial stunning in rat heart. Med Sci Monit 10: BR4-10, 2004.
9. Sekiguchi T, Hosoyama Y, and Miyata S. Effects of proteasome inhibitor (lactacystin) and cysteine protease inhibitor (E-64-d) on processes of mitosis in Xenopus embryonic cells. Zoolog Sci 19: 1251-1255, 2002.
10. Tissot G, Canard H, Nadai M, Martone A, Botterman J, and Dubald M. Translocation of aprotinin, a therapeutic protease inhibitor, into the thylakoid lumen of genetically engineered tobacco chloroplasts. Plant Biotechnol J 6: 309-320, 2008.
    
11. Tsuji S and Matsushita H. Successful treatment of murine muscular dystrophy with the protease inhibitor bestatin. J Neurol Sci 72: 183-194, 1986.
    
12. Vlasova TI and Vanyushin BF. DNA methylation by wheat cytosine DNA methyltransferase: modulation by protease inhibitor E-64. Biochem Mol Biol Int 45: 145-153, 1998.
    

Related Product:  Protease Inhibitor Cocktail with EDTA (100X water soluble)