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InformationSodium Orthovanadate Activated | Vanadate, Na3VO4 | 200 mM Soln | FIVEphoton Biochemicals | Inhibitors | ActVO-4
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Sodium Orthovanadate (Vanadate, Na3VO4)
Pre-Activated, 200 mM, 98% vanadate in dH20, pH 10
Available volumes: 6 ml, 12 ml, 20 ml, 30 ml
Synonyms: Orthovanadate, Vanadate, Sodium Vanadate
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Provided as a pre-activated 200 mM sodium orthovanadate (vanadate) solution. Sodium Orthovanadate is prepared by a pH 10 adjustment and boiling cycle until the solution maintains a stable colorless appearance at equilibrium at pH 10§. This preparation depolymerizes sodium orthovanadate and substantially enhances its potency as an inhibitor of phosphotyrosyl phosphatases.§ Sodium orthovanadate also operates as a potent inhibitor of alkaline phosphatases and Na+K+ATPases due to its chemical properties as a phosphate analog.
§ Gordon, J.: Methods Enzymol. (1991) 201, 477-482.
Huyer, G., et al.: J. Biol. Chem. (1997) 272, 843-851.
Sodium Orthovanadate (Activated) Specifications:
Formula: Na3VO4
CAS Number: 13721-39-6
Purity: 98% vanadate in dH20
Molecular Weight: 183.9
Solubility in water: soluble
Appearance: colorless, clear solution (in dH20, pH 10.0)
Pre-activated through depolymerization at pH 10.0 with a pH-boiling cycle until colorless
Provided concentration: 200 mM
Experimental concentration: 1-10 mM
Storage: Aliquot; -20oC. (After defrosting, vortex to solubilize crystals). Stable at -20oC for at least one year.
Data Sheet 4/2013
Sodium Orthovanadate (Vanadate) Available Volumes:
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Available Volumes*
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Price
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6 ml
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$78.80
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12 ml
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$140.00
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20 ml
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$200.00
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30 ml |
$300.00 |
*Select sodium orthovanadate (vanadate) volumes in Available Options menu.
Sodium Orthovanadate (Vanadate) Applications:
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Maintains tyrosyl phosphorylation in cell lysates and live cell culture (syringe sterilization of solution required for cell culture).
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Alkaline phosphatase and ATPase inhibitor
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Nucleotide ATPase trap, and P-glycoprotein inhibitor
Sodium Orthovanadate (vanadate) is added to cell lysis buffers to preserve protein tyrosine phosphorylation. The preservation of protein phosphorylation by sodium orthovanadate (vanadate) can be detected by signal transduction assay, altered mobility in SDS-PAGE gels, and/or by detection with phospho-specific antibodies. Sodium orthovanadate (vanadate) also has important applicability in ATPase functional assays, including in ABC transporter assays, including P-glycoprotein.
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Directions (Phosphatase Inhibition)
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- Concentration: Recommended experimental sodium orthovanadate (vanadate) concentration for phosphatase inhibition: 1-10 mM.
- Cell lysates: Dispense the 200 mM stock sodium orthovanadate solution directly into cell lysates to make a final concentration of 1-10 mM.
- Cell culture: Syringe sterilize with a 2 micron filter, dispense 200 mM stock solution to cell culture medium to make a final 10 mM concentration.
Images and Data Tab: Sodium Orthovanadate (Vanadate) complexed with alkaline phosphatase. Ref: (1999) J.Biol.Chem. 274: 8351-8354. Source: PDB
Selected references on sodium orthovanadate (vanadate) on phosphatase inhibition and applications to cell signaling research.
- Feng Y, Bhatt AJ, Fratkin JD, and Rhodes PG. Neuroprotective effects of sodium orthovanadate after hypoxic-ischemic brain injury in neonatal rats. Brain Res Bull 76: 102-108, 2008.
- Hasegawa Y, Morioka M, Hasegawa S, Matsumoto J, Kawano T, Kai Y, Yano S, Fukunaga K, and Kuratsu J. Therapeutic time window and dose dependence of neuroprotective effects of sodium orthovanadate following transient middle cerebral artery occlusion in rats. J Pharmacol Exp Ther 317: 875-881, 2006.
- Hulley PA, Conradie MM, Langeveldt CR, and Hough FS. Glucocorticoid-induced osteoporosis in the rat is prevented by the tyrosine phosphatase inhibitor, sodium orthovanadate. Bone 31: 220-229, 2002.
- Kafl HE and Elkashef HA. Effect of sodium orthovanadate on the urinary bladder rings isolated from normal and hyperglycemic rats. Pak J Pharm Sci 19: 195-201, 2006.
- Klein A, Holko P, Ligeza J, and Kordowiak AM. Sodium orthovanadate affects growth of some human epithelial cancer cells (A549, HTB44, DU145). Folia Biol (Krakow) 56: 115-121, 2008.
- Li L, Eisen AZ, Sturman E, and Seltzer JL. Protein tyrosine phosphorylation in signalling pathways leading to the activation of gelatinase A: activation of gelatinase A by treatment with the protein tyrosine phosphatase inhibitor sodium orthovanadate. Biochim Biophys Acta 1405: 110-120, 1998.
- Matsumoto J, Morioka M, Hasegawa Y, Kawano T, Yoshinaga Y, Maeda T, Yano S, Kai Y, Fukunaga K, and Kuratsu J. Sodium orthovanadate enhances proliferation of progenitor cells in the adult rat subventricular zone after focal cerebral ischemia. J Pharmacol Exp Ther 318: 982-991, 2006.
- Milward AD, Riba R, Patel B, Oberprieler NG, and Naseem KM. Sodium orthovanadate induced tyrosine phosphorylation of platelet nitric oxide synthase negatively regulates enzyme activity. Biochim Biophys Acta 1760: 1411-1417, 2006.
- Morita A, Zhu J, Suzuki N, Enomoto A, Matsumoto Y, Tomita M, Suzuki T, Ohtomo K, and Hosoi Y. Sodium orthovanadate suppresses DNA damage-induced caspase activation and apoptosis by inactivating p53. Cell Death Differ 13: 499-511, 2006.
- Pelassy C, Breittmayer JP, and Aussel C. Inhibition of phosphatidylserine synthesis during Jurkat T cell activation. The phosphatase inhibitor, sodium ortho-vanadate bypasses the CD3/T cell receptor-induced second messenger signaling pathway. Eur J Biochem 267: 984-992, 2000.
- Preet A, Siddiqui MR, Taha A, Badhai J, Hussain ME, Yadava PK, and Baquer NZ. Long-term effect of Trigonella foenum graecum and its combination with sodium orthovanadate in preventing histopathological and biochemical abnormalities in diabetic rat ocular tissues. Mol Cell Biochem 289: 137-147, 2006.
- Tran NP, Park JK, Hong SJ, and Lee CG. Proteomics of proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under the influence of sodium orthovanadate. Biotechnol Lett 31: 1917-1922, 2009.
- Ueki H, Yamasaki Y, Higo K, Motoyashiki T, Kawabata H, and Morita T. Regulation of cyclic AMP phosphodiesterase activity by particulate protein tyrosine kinase and phosphotyrosine phosphatase activities sensitive to sodium orthovanadate. Biol Pharm Bull 18: 214-218, 1995.
- Wo YY, Sheu DS, and Lu CH. Sodium vanadate treatment as a shortcut following alkaline phosphatase cleavage. Biotechniques 25: 350-352, 1998.
- Wu DN, Pei DS, Wang Q, and Zhang GY. Down-regulation of PTEN by sodium orthovanadate inhibits ASK1 activation via PI3-K/Akt during cerebral ischemia in rat hippocampus. Neurosci Lett 404: 98-102, 2006.
Sodium Orthovanadate (Vanadate) Storage, Safety, Shipping
Storage: -20oC upon arrival
Safety: Harmful. Avoid ingestion, skin and eye contact.
Shipping: Stable for shipping at ambient temperature: Store at -20oC on arrival. Flat rate Fedex overnight United States delivery $24, 2-day Fedex delivery $16.00 first bottle. Free shipping for this item to academic, biotech and pharma research institutions in California and Arizona. Use coupon code freeship on online checkout. International priority Fedex delivery is also available.
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