Sodium Orthovanadate (Vanadate) Pre-Activated 200mM Soln | Na3VO4 | Part No. ActVO-4

Sodium Orthovanadate (Vanadate) Pre-Activated 200mM Soln | Na3VO4 | Part No. ActVO-4
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Price: $78.80
Availability: In Stock
Model: ActVO-4
Manufacturer: FIVEphoton Biochemicals

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Sodium Orthovanadate (Vanadate, Na3VO4)
Pre-activated 200 mM solution 
  • Ready-to-use, pre-activated, 200 mM solution in dH20, pH10. 
  • Starting material is >99% vanadate solid. 
  • Formulated by depolymerization at pH 10.0 with a pH-boiling cycle until colorless at equilibrium.
  • Potent tyrosyl and alkaline phosphatase inhibitor. 
  • Dispense directly into cell lysates.
Synonyms:  Orthovanadate, Vanadate, Sodium Vanadate
 
Availability:  Ships the same day for orders received before 1:30 PM Mon-Thurs.  Orders on Friday are shipped on Monday. 

 
    Available Volumes
     Price     
6 ml
$78.80
12 ml
$140.00






Additional  Volumes
    Volume
     Price     
20 ml
$188.00
30 ml
$270.00



 

Specifications:

  • Formula:  Na3VO4
  • CAS Number:  13721-39-6
  • Provided concentration:  200 mM
  • Purity:  Starting material:  >99% vanadate solid stock
  • Molecular Weight:  183.9
  • Solubility in water:  soluble
  • Appearance:  colorless, clear solution (in dH20, pH10)
  • Pre-activated through depolymerization at pH 10.0 with a pH-boiling cycle until colorless at equilibrium
  • Experimental concentration:  1 mM
  • Inhibition reversed by EDTA, DTT
  • Shipped at ambient temperature
  • Storage:  Aliquot; -20oC.  (After defrosting, vortex to solubilize crystals).  Stable at -20oC for at least one year.
  Data Sheet


 
Highlights
 
Sodium Orthovanadate  (Part ACTVO-4) is provided as a potent inhibitor that has been pre-activated by a pH10 adjustment and boiling cycle until the solution maintains a colorless appearance at equilibrium at pH10§.  This preparation depolymerizes sodium orthovanadate and enhances its potency as an inhibitor of phosphotyrosyl phosphatases.§ 

This activated sodium orthovanadate solution is diluted directly into cell lysates or cell culture medium.  Pre-activated sodium orthovanadate solution is stable for at least one year when stored at -20oC.   Certain buffer components such as EDTA and oxidants interact with inhibition potency:  HEPES is a preferred buffer to maintain vanadate potency.2

Reference on formulation:
§  1.  Gordon, J.:  Methods Enzymol. (1991) 201, 477-482.

Reference on mechanism of inhibition and assay buffer interactions:
    2.  Huyer, G., et al.:  J. Biol. Chem. (1997) 272, 843-851.

Sodium Orthovanadate (Vanadate) Applications:

  1. Maintains tyrosyl phosphorylation in cell lysates and live cell culture (syringe sterilization of solution required for cell culture).
  2. Alkaline phosphatase inhibitor
  3. ATPase inhibitor
  4. Use as a stock solution for pervanadate generation


  Directions (Phosphatase Inhibition)  
  1. Concentration:  Recommended experimental sodium orthovanadate (vanadate) concentration for phosphatase inhibition is 1 mM. 
  2. Cell lysates:  Dispense the 200 mM stock sodium orthovanadate solution directly into cell lysates to make a final concentration of 1 mM. 
  3. Cell culture:  Syringe sterilize with a 2 micron filter, dispense the 200 mM stock solution to cell culture medium to make a final 1 mM concentration.

Images and Data Tab: Sodium Orthovanadate (Vanadate) complexed with alkaline phosphatase. Ref: (1999) J.Biol.Chem. 274: 8351-8354. Source: PDB

Product Reference
 
Bunda, S et.al. 2014.  PNAS Plus: Src promotes GTPase activity of Ras via tyrosine 32 phosphorylation PNAS 2014 111 (36) E3785-E3794; published ahead of print August 25, 2014.


Related Product of Interest:  Highly sensitive cell counting, cell proliferation and cell death assay that uses WST-8 reagent.

Fivephoton Cell Viability and Proliferation Assay Kit:  click here to view webpage

 

Selected references on sodium orthovanadate (vanadate) on phosphatase inhibition and applications to cell signaling research.
  1. Feng Y, Bhatt AJ, Fratkin JD, and Rhodes PG. Neuroprotective effects of sodium orthovanadate after hypoxic-ischemic brain injury in neonatal rats Brain Res Bull 76: 102-108, 2008.
  2. Hasegawa Y, Morioka M, Hasegawa S, Matsumoto J, Kawano T, Kai Y, Yano S, Fukunaga K, and Kuratsu J. Therapeutic time window and dose dependence of neuroprotective effects of sodium orthovanadate following transient middle cerebral artery occlusion in rats. J Pharmacol Exp Ther 317: 875-881, 2006.
  3. Hulley PA, Conradie MM, Langeveldt CR, and Hough FS. Glucocorticoid-induced osteoporosis in the rat is prevented by the tyrosine phosphatase inhibitor, sodium orthovanadate. Bone 31: 220-229, 2002.
  4. Kafl HE and Elkashef HA. Effect of sodium orthovanadate on the urinary bladder rings isolated from normal and hyperglycemic rats. Pak J Pharm Sci 19: 195-201, 2006.
  5. Klein A, Holko P, Ligeza J, and Kordowiak AM. Sodium orthovanadate affects growth of some human epithelial cancer cells (A549, HTB44, DU145). Folia Biol (Krakow) 56: 115-121, 2008.
  6. Li L, Eisen AZ, Sturman E, and Seltzer JL. Protein tyrosine phosphorylation in signalling pathways leading to the activation of gelatinase A: activation of gelatinase A by treatment with the protein tyrosine phosphatase inhibitor sodium orthovanadate. Biochim Biophys Acta 1405: 110-120, 1998.
  7. Matsumoto J, Morioka M, Hasegawa Y, Kawano T, Yoshinaga Y, Maeda T, Yano S, Kai Y, Fukunaga K, and Kuratsu J. Sodium orthovanadate enhances proliferation of progenitor cells in the adult rat subventricular zone after focal cerebral ischemia. J Pharmacol Exp Ther 318: 982-991, 2006.
  8. Milward AD, Riba R, Patel B, Oberprieler NG, and Naseem KM. Sodium orthovanadate induced tyrosine phosphorylation of platelet nitric oxide synthase negatively regulates enzyme activity. Biochim Biophys Acta 1760: 1411-1417, 2006.
  9. Morita A, Zhu J, Suzuki N, Enomoto A, Matsumoto Y, Tomita M, Suzuki T, Ohtomo K, and Hosoi Y. Sodium orthovanadate suppresses DNA damage-induced caspase activation and apoptosis by inactivating p53. Cell Death Differ 13: 499-511, 2006.
  10. Pelassy C, Breittmayer JP, and Aussel C. Inhibition of phosphatidylserine synthesis during Jurkat T cell activation. The phosphatase inhibitor, sodium ortho-vanadate bypasses the CD3/T cell receptor-induced second messenger signaling pathway. Eur J Biochem 267: 984-992, 2000.
  11. Preet A, Siddiqui MR, Taha A, Badhai J, Hussain ME, Yadava PK, and Baquer NZ. Long-term effect of Trigonella foenum graecum and its combination with sodium orthovanadate in preventing histopathological and biochemical abnormalities in diabetic rat ocular tissues. Mol Cell Biochem 289: 137-147, 2006.
  12. Tran NP, Park JK, Hong SJ, and Lee CG. Proteomics of proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under the influence of sodium orthovanadate. Biotechnol Lett 31: 1917-1922, 2009.
  13. Ueki H, Yamasaki Y, Higo K, Motoyashiki T, Kawabata H, and Morita T. Regulation of cyclic AMP phosphodiesterase activity by particulate protein tyrosine kinase and phosphotyrosine phosphatase activities sensitive to sodium orthovanadate. Biol Pharm Bull 18: 214-218, 1995.
  14. Wo YY, Sheu DS, and Lu CH. Sodium vanadate treatment as a shortcut following alkaline phosphatase cleavage. Biotechniques 25: 350-352, 1998.
  15. Wu DN, Pei DS, Wang Q, and Zhang GY. Down-regulation of PTEN by sodium orthovanadate inhibits ASK1 activation via PI3-K/Akt during cerebral ischemia in rat hippocampus. Neurosci Lett 404: 98-102, 2006. 

  
Sodium Orthovanadate (Vanadate) Storage, Safety, Shipping
Storage: Ships at ambient temperature.  Store at -20oC upon arrival.  Stable for at least one year after arrival at -20oC.
Safety:  Harmful.  Avoid ingestion, skin and eye contact. 
Shipping:   2-day or overnight delivery recommended.  International priority Fedex delivery is also available. 

  


 

Category Download Link
Specification Sheet
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MSDS click here



 

Sodium Orthovanadate (Vanadate) Pre-Activated 200mM Soln | Na3VO4 | Part No. ActVO-4
Click to enlarge
Sodium Orthovanadate (Vanadate) Pre-Activated 200mM Soln | Na3VO4 | Part No. ActVO-4
Click to enlarge