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Protein Extraction Reagents

Protein Extraction, Cell Lysis and Immunoprecipitation Reagents
 
Detergent protein extraction reagents lyse and soubilize cell menbranes, a process that releases proteins into solution into a detergent buffer.  All protein extraction reagents offered by FIVEphoton Biochemicals extract transmembrane and cytosolic proteins from culture cells;  some are also suitable to extract proteins from tissues.  We also offer protein extraction reagents that can be used in immunoprecipitation and pull-down procedures (for example, the CHAPS lysis buffer).  
 
The Transmembrane Protein Extraction Reagent (TmPER -) will extract and solubilize high molecular weight - multipass transmembrane proteins that are poorly soluble, have aggregation tendency and/or are lipid raft associated.  The table below overviews properties of our protein extraction reagents.

Overview of Protein Extraction Reagents

Reagent Part No. Extacts high molecular weight transmembrane proteins

Maintains native conformation
and protein interactions

 For IP and ELISA Lipid raft extraction Strong extraction Mild extraction
Transmembrane Protein Extraction Reagent tmPER x

x
 x
CHAPS  Cell Lysis and Immunoprecipitation Buffer CIB-1
x x
x
Tissue Protein Extraction Buffer TEB-1
x
 x

x
Immunoprecipitation Bufffer With Triton TIB-1
x x

x
ELISA Lysis and Protein Extraction Buffer
 ELSP-1
x
 x

x






 
 
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CHAPS IMMUNOPRECIPITATION (IP) AND LYSIS BUFFER For Tissues and Cells | Part No. CIB-1
CHAPS IMMUNOPRECIPITATION (IP) AND LYSIS BUFFER For Tissues and Cells | Part No. CIB-1
CIB-1
$165.00
 ELISA PROTEIN EXTRACTION, ISOLATION & SOLUBILIZATION BUFFER FOR ELISA SAMPLES (Part No. ELSP-1)
ELISA PROTEIN EXTRACTION, ISOLATION & SOLUBILIZATION BUFFER FOR ELISA SAMPLES (Part No. ELSP-1)
ELSP-1
$120.00
 Immunoprecipitation Buffer w/ Triton X-100 (TIB-1)
Immunoprecipitation Buffer w/ Triton X-100 (TIB-1)
TIB-1
$145.00
 PROTEIN EXTRACTION - CELL LYSIS BUFFERS (Parts TMPER, CIB-1, ELSP-1, TEB-1)
PROTEIN EXTRACTION - CELL LYSIS BUFFERS (Parts TMPER, CIB-1, ELSP-1, TEB-1)
Prot-Extrct
$79.00
RIPA Buffer (RIPA-50)
RIPA Buffer (RIPA-50)
RIPA-50
$82.00
 Tissue Protein Extraction Buffer (1X, 100 ml; Part No TEB-1)
Tissue Protein Extraction Buffer (1X, 100 ml; Part No TEB-1)
TEB-1
$125.00
 Translocation Assay Buffers
Translocation Assay Buffers
TAB-1
$120.00
 Transmembrane Protein Extraction Reagent (Part No. tmPER-50; Plasma Protein Isolation)
Transmembrane Protein Extraction Reagent (Part No. tmPER-50; Plasma Protein Isolation)
TmPER-50 (100 plates)
$180.00
TRANSMEMBRANE PROTEIN EXTRACTION REAGENT (tmPER-100; Membrane Protein Isolation)
TRANSMEMBRANE PROTEIN EXTRACTION REAGENT (tmPER-100; Membrane Protein Isolation)
TmPER-100 (150 plates)
$200.00
 Transmembrane Protein Extraction Reagent (tmPER-200; Plasma Protein Isolation)
Transmembrane Protein Extraction Reagent (tmPER-200; Plasma Protein Isolation)
TmPER-200 (300 plates)
$260.00